Stress-induced breakdown of intestinal barrier function in the rat: reversal by wood creosote

Life Sciences
Tomoo KugeMasahiro Sokabe

Abstract

Our previous studies demonstrated that wood creosote (Seirogan) inhibits intestinal secretion and normalizes the transport of electrolytes and water in rats subjected to restraint stress. The goal of the present study was to examine whether wood creosote has a protective effect against stress-induced breakdown of intestinal barrier function. F-344 rats were subjected to 90-min water avoidance stress (WAS) with wood creosote (30 mg/kg) or vehicle administered intragastrically 30 min prior to WAS. Sham stressed rats received wood creosote or vehicle treatment but did not experience the WAS. All rats were euthanized at the end of the WAS or sham-stress and the jejunum and colon were isolated. Epithelial transport was studied in modified Ussing chambers. Spontaneous secretion was assessed by electrophysiological measurement of the short circuit current (I(sc)) while electrical conductance (G) was calculated from the potential difference (PD) and I(sc) using Ohm's law. Intestinal permeability was defined by the mucosal-to-serosal flux of horseradish peroxidase (HRP). WAS significantly elevated basal I(sc) and G and increased epithelial permeability to HRP in the jejunum but not in the colon. Wood creosote resulted in a significant r...Continue Reading

References

Jan 20, 1999·Alimentary Pharmacology & Therapeutics·B Greenwood-Van MeerveldN Ogata
Aug 30, 2000·Biological & Pharmaceutical Bulletin·B Greenwood-Van MeerveldT Kuge
Mar 5, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sylvie BradesiEmeran A Mayer

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Citations

Apr 30, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·E JorgeM T Martin
Dec 30, 2014·Journal of Neurogastroenterology and Motility·Bruno K Rodiño-JaneiroJavier Santos
Apr 10, 2010·Journal of Biomedicine & Biotechnology·Takahiko ShiinaYasutake Shimizu
Apr 4, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·T VanhaeckeM Neunlist

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