Stress-induced pseudocyst formation--a newly identified mechanism of protection against organic solvents in acanthamoebae of the T4 genotype

Protist
Jarmila KliescikovaEva Nohynkova

Abstract

Differentiation into highly resistant double-walled cysts is a major mechanism allowing amphizoic acanthamoebae to survive under long-lasting, unfavourable environmental conditions. We found that relatively low concentrations of methanol, acetone or DMSO stimulate promptAcanthamoebadifferentiation into a rounded cyst-like stage with a single envelope. To address whether this rapid response differs from the encystment, time-dependent changes in cell surface characteristics and cyst-specific gene expression were monitored in encystating cells and cells differentiating under methanol treatment using microscopic, lectin-binding, PCR and resistance studies. In contrast to the encystment: (1) a single-layered amorphous mannose/glucose coat was the only envelope assembled on the surface of the solvent-treated cells, (2) the cyst-specific protein (CSP21) was not expressed, (3) the coat did not protect cells against acidic pH and (4) in solvent-free encystment medium, the coated cells did not assemble the double-layered wall, thus indicating that these cells were not immature cysts. These findings lead us to specify a terminal stage of rapidAcanthamoebadifferentiation elicited by acute organic solvent stress as "pseudocyst", and to sugg...Continue Reading

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Citations

Dec 25, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Fu-Chin HuangWei-Chen Lin
Aug 24, 2010·Experimental Parasitology·Jarmila KliescikovaEva Nohynkova
May 12, 2012·Molecular and Biochemical Parasitology·Eva BínováEva Nohýnková
Feb 5, 2015·BioMed Research International·Naveed Ahmed KhanRuqaiyyah Siddiqui
May 3, 2016·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Ruqaiyyah SiddiquiNaveed Ahmed Khan
Aug 14, 2020·Parasitology Research·Daniele Corsaro
Jun 9, 2020·International Journal for Parasitology. Drugs and Drug Resistance·Lenu B FakaeHany M Elsheikha
Nov 12, 2021·Parasite : Journal De La Société Française De Parasitologie·Jian-Ming HuangFu-Chin Huang

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