Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii.

The Journal of Cell Biology
James Uniacke, William Zerges

Abstract

Eukaryotic cells under stress repress translation and localize these messenger RNAs (mRNAs) to cytoplasmic RNA granules. We show that specific stress stimuli induce the assembly of RNA granules in an organelle with bacterial ancestry, the chloroplast of Chlamydomonas reinhardtii. These chloroplast stress granules (cpSGs) form during oxidative stress and disassemble during recovery from stress. Like mammalian stress granules, cpSGs contain poly(A)-binding protein and the small, but not the large, ribosomal subunit. In addition, mRNAs are in continuous flux between polysomes and cpSGs during stress. Localization of cpSGs within the pyrenoid reveals that this chloroplast compartment functions in this stress response. The large subunit of ribulosebisphosphate carboxylase/oxygenase also assembles into cpSGs and is known to bind mRNAs during oxidative stress, raising the possibility that it plays a role in cpSG assembly. This discovery within such an organelle suggests that mRNA localization to granules during stress is a more general phenomenon than currently realized.

References

Sep 1, 1989·Molecular and Cellular Biology·E D von GromoffC F Beck
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·C B YohnS P Mayfield
Dec 21, 2000·The Journal of Cell Biology·N KedershaP Anderson
Nov 21, 2002·Biochemical Society Transactions·N Kedersha, P Anderson
Jun 22, 2005·The Journal of Cell Biology·Nancy KedershaPaul Anderson
Sep 7, 2005·Photosynthesis Research·María Verónica BeligniStephen P Mayfield
Jan 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·N Sueoka
Jun 21, 2006·Biochimica Et Biophysica Acta·Yoshitaka NishiyamaNorio Murata
Nov 15, 2006·The Journal of Cell Biology·Nadine StöhrStefan Hüttelmaier
Jan 9, 2007·Biochimica Et Biophysica Acta·Norio MurataSuleyman I Allakhverdiev
Mar 14, 2007·Molecular Cell·Roy Parker, Ujwal Sheth
Dec 7, 2007·The Plant Cell·James Uniacke, William Zerges
Feb 23, 2008·Trends in Biochemical Sciences·Paul Anderson, Nancy Kedersha

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Citations

Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·James Uniacke, William Zerges
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marco SchottkowskiWilliam Zerges
Nov 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·Juan Carlos Villarreal, Susanne S Renner
May 5, 2012·American Journal of Physiology. Cell Physiology·Tongtong ZouJian-Ying Wang
Sep 29, 2011·Microbial Cell Factories·Mireia Giménez-Barcons, Juana Díez
Nov 26, 2010·RNA·Daniel Nilsson, Per Sunnerhagen
Dec 18, 2013·Antioxidants & Redox Signaling·Samuel MorisseChristophe H Marchand
Mar 10, 2016·The Journal of Cell Biology·Alexis A JourdainJean-Claude Martinou
Jun 1, 2011·The Plant Journal : for Cell and Molecular Biology·Stephan EberhardFrancis-André Wollman
Mar 26, 2010·Proteomics·Zeina DaherEliane Dumas-Gaudot
Mar 15, 2015·The Plant Journal : for Cell and Molecular Biology·Yingjun WangMartin H Spalding
Jan 24, 2015·Biochimica Et Biophysica Acta·Anna RastJörg Nickelsen
Sep 4, 2010·Cellular Signalling·María Gabriela ThomasGraciela Lidia Boccaccio
May 27, 2009·Current Biology : CB·Paul Anderson, Nancy Kedersha
Jun 21, 2014·Seminars in Cell & Developmental Biology·Pauline Adjibade, Rachid Mazroui
Feb 22, 2018·Antioxidants & Redox Signaling·Karl-Josef DietzThorsten Seidel
May 24, 2020·Wiley Interdisciplinary Reviews. RNA·Nisansala S MuthunayakeJared M Schrader
Jul 18, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anne-Marie LadouceurStephanie C Weber
Dec 1, 2020·Plant Physiology and Biochemistry : PPB·Annelie MarquardtFrederik C Botha
Jan 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Drago LauraSantovito Gianfranco
Oct 10, 2015·Journal of Cell Science·Yu ZhanWilliam Zerges
Apr 21, 2021·Current Opinion in Microbiology·Vidhyadhar Nandana, Jared M Schrader
Jul 23, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Semen V NesterovVladimir N Uversky
Aug 27, 2021·Frontiers in Plant Science·Israel Maruri-LópezMonika Chodasiewicz
Nov 19, 2021·Yeast·Tomas GrouslTomas Vomastek

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Basic Local Alignment Search Tool
ImageJ
Leica

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