Stress regulated members of the plant organic cation transporter family are localized to the vacuolar membrane.

BMC Research Notes
Isabell Küfner, Wolfgang Koch

Abstract

In Arabidopsis six genes group into the gene family of the organic cation transporters (OCTs). In animals the members of the OCT-family are mostly characterized as polyspecific transporters involved in the homeostasis of solutes, the transport of monoamine neurotransmitters and the transport of choline and carnitine. In plants little is known about function, localisation and regulation of this gene family. Only one protein has been characterized as a carnitine transporter at the plasma membrane so far. We localized the five uncharacterized members of the Arabidopsis OCT family, designated OCT2-OCT6, via GFP fusions and protoplast transformation to the tonoplast. Expression analysis with RNA Gel Blots showed a distinct, organ-specific expression pattern of the individual genes. With reporter gene fusion of four members we analyzed the tissue specific distribution of OCT2, 3, 4, and 6. In experiments with salt, drought and cold stress, we could show that AtOCT4, 5 and 6 are up-regulated during drought stress, AtOCT3 and 5 during cold stress and AtOCT 5 and 6 during salt stress treatments. Localisation of the proteins at the tonoplast and regulation of the gene expression under stress conditions suggests a specific role for the tr...Continue Reading

References

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May 25, 2007·The Plant Journal : for Cell and Molecular Biology·Christine Lelandais-BrièreCaroline Hartmann

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Citations

Jan 24, 2014·Oxidative Medicine and Cellular Longevity·Shuchita TiwariJoseph Fomusi Ndisang
Jan 15, 2011·Science·Jeffrey Kiehl
Oct 18, 2019·Plant Molecular Biology·Mst Hur MadinaHugo Germain
Feb 21, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Pauline van HaperenBen Vosman
Apr 10, 2021·BMC Plant Biology·Lidia López-SerranoEduardo Bueso

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Methods Mentioned

BETA
RNA-gel
salt
salt stress
X-ray

Software Mentioned

aramemnon

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