Stress resistance and signal fidelity independent of nuclear MAPK function.

Proceedings of the National Academy of Sciences of the United States of America
Patrick J WestfallJeremy Thorner

Abstract

Elevated external solute stimulates a conserved MAPK cascade that elicits responses that maintain osmotic balance. The yeast high-osmolarity glycerol (HOG) pathway activates Hog1 MAPK (mammalian ortholog p38alpha/SAPKalpha), which enters the nucleus and induces expression of >50 genes, implying that transcriptional up-regulation is necessary to cope with hyperosmotic stress. Contrary to this expectation, we show here that cells lacking the karyopherin required for Hog1 nuclear import or in which Hog1 is anchored at the plasma membrane (or both) can withstand long-term hyperosmotic challenge by ionic and nonionic solutes without exhibiting the normal change in transcriptional program (comparable with hog1Delta cells), as judged by mRNA hybridization and microarray analysis. For such cells to survive hyperosmotic stress, systematic genetic analysis ruled out the need for any Hog1-dependent transcription factor, the Hog1-activated MAPKAP kinases, or ion, glycerol, and water channels. By contrast, enzymes needed for glycerol production were essential for viability. Thus, control of intracellular glycerol formation by Hog1 is critical for maintenance of osmotic balance but not transcriptional induction of any gene.

References

Feb 15, 1988·Analytical Biochemistry·R N Skopp, L C Lane
Jan 1, 1987·Methods in Enzymology·J D BoekeG R Fink
Oct 5, 1984·Journal of Molecular Biology·M SternI Herskowitz
May 11, 2000·Molecular and Cellular Biology·E Bilsland-MarchesanF Posas
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·M TeigeG Ammerer
Dec 17, 2002·Molecular and Cellular Biology·Eulàlia de NadalFrancesc Posas
Nov 5, 2003·Molecular Biology of the Cell·Sean M O'Rourke, Ira Herskowitz
Feb 7, 2004·European Journal of Biochemistry·Sara KarlgrenStefan Hohmann
Jul 23, 2004·Molecular Biology of the Cell·Philippe Kieffer-KwonIrwin Davidson
Nov 4, 2004·American Journal of Physiology. Renal Physiology·David Sheikh-Hamad, Michael C Gustin
Nov 30, 2004·Science·Patrick J WestfallJeremy Thorner
Jun 22, 2005·Biology of the Cell·Nina PetterssonStefan Hohmann
Jul 29, 2006·Science·Dmitry K PokholokRichard A Young
Aug 4, 2006·Molecular Biology of the Cell·Michael ThorsenMarkus J Tamás
Mar 10, 2007·FEBS Letters·Sungjoon Kim, Kavita Shah
Jul 3, 2007·Biochimica Et Biophysica Acta·Raymond E Chen, Jeremy Thorner
Jan 26, 2008·Science·Jerome T MettetalAlexander van Oudenaarden

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Citations

Mar 25, 2011·Biotechnology Letters·Xiao-Xong CaoLi-Hua Hou
Nov 4, 2011·Nature Reviews. Genetics·Eulàlia de NadalFrancesc Posas
Oct 16, 2008·The Journal of Biological Chemistry·Nan HaoHenrik G Dohlman
May 1, 2010·The Journal of Biological Chemistry·Francisco Torres-QuirozJose A Prieto
Apr 13, 2010·The Journal of Biological Chemistry·Alicia A BicknellMaho Niwa
Jun 12, 2012·The Journal of Biological Chemistry·Laura Sánchez-MirJosé Cansado
Aug 11, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Elias August
Jul 1, 2010·Molecular Biology of the Cell·Jonas WarringerPer Sunnerhagen
Apr 25, 2013·Science Signaling·Rodrigo BaltanásAlejandro Colman-Lerner
Jul 5, 2011·Applied and Environmental Microbiology·Georg HubmannElke Nevoigt
Jun 14, 2013·PLoS Computational Biology·Elzbieta Petelenz-KurdzielEdda Klipp
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Jun 4, 2011·Journal of Signal Transduction·Agnès MiermontPascal Hersen
Sep 18, 2014·Science Signaling·Jay L Brewster, Michael C Gustin
Aug 28, 2012·Trends in Endocrinology and Metabolism : TEM·Jason W Locasale
Mar 2, 2016·The Journal of Cell Biology·Galo GarciaJeremy Thorner
Nov 20, 2010·Current Opinion in Biotechnology·Miguel C TeixeiraIsabel Sá-Correia
Mar 15, 2016·Metabolic Engineering·Steve SwinnenElke Nevoigt
Jan 28, 2009·Cellular Microbiology·Lukasz KozubowskiJoseph Heitman
Mar 7, 2013·Molecular Microbiology·Kentaro Furukawa, Stefan Hohmann

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