Stress response of Escherichia coli to essential oil components - insights on low-molecular-weight proteins from MALDI-TOF

Scientific Reports
Matěj BožikPavel Klouček

Abstract

The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on low-molecular-weight proteins. From 297 m/z features, we identified 94 proteins with important differences in expression among untreated samples, samples treated with EOCs, and samples treated with antibiotics, peroxide, or chlorine. The targets of these treatments obviously differ, even among EOCs. In addition to ribosomal proteins, stress-, membrane- and biofilm-related proteins were affected. These findings may provide a basis for identifying new targets of essential oils and synergies with other antibiotics.

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Citations

Feb 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mariam Nasser AljaafariSwee-Hua Erin Lim
Dec 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonio MonopoliCosima D Calvano
Aug 11, 2021·Plants·Lucia GalovičováMiroslava Kačániová

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Methods Mentioned

BETA
electrophoresis
PCA

Software Mentioned

- toolbox
Mass Spectrum Analysis and Data Conversion Tool
TrEMBL
flexControl
hclust
project multiMS - toolbox
multiMS
R
MALDI Biotyper
FlexAnalysis

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