Stress Response Simulated by Continuous Injection of ACTH Attenuates Lipopolysaccharide-Induced Inflammation in Porcine Adrenal Gland

Frontiers in Veterinary Science
Zhiyuan SunXuhui Zhang

Abstract

On modern farms, animals are at high risk of bacterial invasion due to environmental stress factors. The adrenal gland is the terminal organ of the stress response. The crosstalk between adrenal endocrine stress and innate immune response is critical for the maintenance of immune homeostasis during inflammation. Thus, it's important to explore whether stresses play a pivotal role in lipopolysaccharide (LPS)-induced inflammatory response in the porcine adrenal gland. Thirty-days-old Duroc × Landrace × Large White crossbred piglets (12 ± 0.5 kg) were randomly allocated into four groups in a 2 × 2 factorial arrangement of treatments, including ACTH pretreatment (with or without ACTH injection) and LPS challenge (with or without LPS injection). Each group consisted of six male piglets. The results showed that our LPS preparation alone induced mRNA expressions of IL-1β, IL-6, TNF-α, IL-10, COX-2, TLR2, TLR4, and GR (P < 0.05). ACTH pretreatment downregulated the TLR2 mRNA and IL-6 protein level induced by our LPS preparation significantly (P < 0.05) by one-way ANOVA analysis. Treatment with LPS alone extremely significantly decreased ssc-miR-338 levels (P < 0.01). Interaction of ACTH × LPS was significant for cNOS level (P = 0.011) ...Continue Reading

References

Jun 1, 1988·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A GamalloM P Nava
Oct 24, 1998·Domestic Animal Endocrinology·R L MatteriR W Johnson
May 14, 1999·The Journal of Clinical Endocrinology and Metabolism·S R Bornstein, G P Chrousos
Jun 7, 2003·Science·Gregory M Barton, Ruslan Medzhitov
Jul 10, 2004·Essays in Biochemistry·William B PrattPatrick J M Murphy
Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Stefan R BornsteinKai Zacharowski
May 12, 2005·Journal of Neuroimmunology·Ru ChenSulie L Chang
Apr 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kai ZacharowskiStefan R Bornstein
Aug 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Konstantin D TaganovDavid Baltimore
Aug 5, 2006·Annals of the New York Academy of Sciences·Y JuarranzR P Gomariz
Nov 7, 2006·Journal of Animal Science·J L Salak-Johnson, J J McGlone
Jun 20, 2007·Molecular and Cellular Endocrinology·Yurii Chinenov, Inez Rogatsky
Oct 14, 2008·Brain, Behavior, and Immunity·Nicole D PowellJohn F Sheridan
Jul 29, 2010·The Journal of Biological Chemistry·Lien DejagerClaude Libert
Oct 4, 2011·Veterinary Microbiology·Elin VerbruggheFrank Pasmans
Dec 27, 2011·Research in Veterinary Science·E TrevisiG Bertoni
Mar 8, 2013·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·S PanX Yang
Aug 10, 2013·Bioinformation·Paul Shapshak
Mar 1, 2014·The Journal of Endocrinology·Saadia BasharatChristopher D John
Jun 20, 2015·International Journal of Biometeorology·Monica BattiniSilvana Mattiello
Mar 1, 2016·Veterinary Immunology and Immunopathology·Massimo Amadori, Cinzia Zanotti
Jul 20, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Meng QiJinyong Peng
Jan 25, 2017·Animal : an International Journal of Animal Bioscience·M J AlcaldeT F Martínez
Jul 23, 2019·Journal of Animal Physiology and Animal Nutrition·Yulong HeHanchuan Dai

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