Stressosomes formed in Bacillus subtilis from the RsbR protein of Listeria monocytogenes allow σ(B) activation following exposure to either physical or nutritional stress

Journal of Bacteriology
Luis MartinezW G Haldenwang

Abstract

The general stress regulon of Bacillus subtilis is controlled by σ(B), a transcription factor that is activated by physical or nutritional stress. In B. subtilis, each of these two stresses is communicated to the primary σ(B) regulators by distinct pathways. Physical stress activation of σ(B) involves a large-molecular-mass (>10(6)-Da) structure (stressosome) formed by one or more homologous proteins (RsbRA, -B, -C, and -D) onto which the pathway's principal regulators are bound. The RsbR proteins are thought to be potential receptors for stress signaling. Listeria monocytogenes encodes orthologs of σ(B) and its principal regulators; however, unlike B. subtilis, L. monocytogenes appears to use the stressosome pathway for both physical and nutritional stress activation of σ(B). In the current work, a B. subtilis strain that expressed L. monocytogenes rsbR (rsbR(Lm)) in lieu of B. subtilis rsbR (rsbR(Bs)) was created and was found to display the Listeria phenotype of σ(B) activation following exposure to either physical or nutritional stress. B. subtilis expressing either the RsbR paralog rsbRC or rsbRD, but not rsbRA or rsbRB, as the sole source of RsbR also allowed σ(B) induction following nutritional stress. It is unclear whet...Continue Reading

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Citations

Jan 17, 2013·BMC Systems Biology·Ulf W LiebalOlaf Wolkenhauer
Feb 28, 2019·Molecular Omics·Alexandra KhitunSarah A Slavoff
Apr 30, 2019·Environmental Microbiology·Nicolas KintIsabelle Martin-Verstraete
Jul 21, 2015·Photochemistry and Photobiology·Jeroen B van der Steen, Klaas J Hellingwerf
Jun 26, 2012·The FEBS Journal·Stefan FuchsAlois Schweighofer
Feb 6, 2021·World Journal of Microbiology & Biotechnology·Hoai T Tran, Carla Y Bonilla

Related Concepts

SigB protein, Bacteria
RsbR protein, Bacillus subtilis
Natto Bacteria
Bacterial Proteins
Cis-Trans Test
Listeria monocytogenes
Phosphoproteins
Proteins, Recombinant DNA
RNA Polymerase Sigma Factor H
Gene Expression

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