Stretch-activated pore of the antimicrobial peptide, magainin 2

Langmuir : the ACS Journal of Surfaces and Colloids
Mohammad Abu Sayem KaralMasahito Yamazaki

Abstract

Antimicrobial peptide magainin 2 forms pores in lipid membranes and induces membrane permeation of the cellular contents. Although this permeation is likely the main cause of its bactericidal activity, the mechanism of pore formation remains poorly understood. We therefore investigated in detail the interaction of magainin 2 with lipid membranes using single giant unilamellar vesicles (GUVs). The binding of magainin 2 to the lipid membrane of GUVs increased the fractional change in the area of the membrane, δ, which was proportional to the surface concentration of magainin 2, X. This indicates that the rate constant of the magainin 2-induced two-state transition from the intact state to the pore state greatly increased with an increase in δ. The tension of a lipid membrane following aspiration of a GUV also activated magainin 2-induced pore formation. To reveal the location of magainin 2, the interaction of carboxyfluorescein (CF)-labeled magainin 2 (CF-magainin 2) with single GUVs containing a water-soluble fluorescent probe, AF647, was investigated using confocal microscopy. In the absence of tension due to aspiration, after the interaction of magainin 2 the fluorescence intensity of the GUV rim due to CF-magainin 2 increased...Continue Reading

References

Jun 1, 1976·Biophysical Journal·E A EvansL Melnik
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D WadeR B Merrifield
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M ZasloffH C Chen
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff
Jan 1, 1993·European Biophysics Journal : EBJ·M S Sansom
Dec 1, 1993·Protein Science : a Publication of the Protein Society·B BechingerS J Opella
Oct 1, 1996·Nature Structural Biology·W C Wimley, S H White
Oct 29, 1996·Biochemistry·S J LudtkeH W Huang
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·O SandreF Brochard-Wyart
Jun 27, 2000·Biophysical Journal·W RawiczE Evans
Oct 12, 2000·Biophysical Journal·L YangH W Huang
Jan 25, 2002·Nature·Michael Zasloff
Jul 3, 2002·Chemistry and Physics of Lipids·Philippe F DevauxPaulette Hervé
Sep 26, 2003·Biophysical Journal·Evan EvansWieslawa Rawicz
Mar 24, 2004·Biochemistry·Ming-Tao LeeHuey W Huang
Jun 1, 2004·Physical Review Letters·Huey W HuangMing-Tao Lee
Jul 1, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Tomoki Tanaka, Masahito Yamazaki
Sep 14, 2006·Journal of the American Chemical Society·Hari LeontiadouSiewert J Marrink
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ming-Tao LeeHuey W Huang
Mar 10, 2009·The Journal of Physical Chemistry. B·Yukihiro Tamba, Masahito Yamazaki
Feb 6, 2010·Physiology·Frederick Sachs
Apr 2, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Tatiana M DominguesAntonio Miranda
Aug 31, 2010·The Journal of Physical Chemistry. B·Yukihiro TambaMasahito Yamazaki
Mar 29, 2011·European Biophysics Journal : EBJ·Gustavo FuertesJesús Salgado
Apr 29, 2011·Chemistry and Physics of Lipids·Yukihiro TambaMasahito Yamazaki
Mar 12, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Victor LevadnyMasahito Yamazaki
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicholas B Last, Andrew D Miranker
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ming-Tao LeeHuey W Huang
Oct 25, 2013·Journal of the American Chemical Society·Sterling A WheatenPaulo F Almeida
May 2, 2014·Biochimica Et Biophysica Acta·Salome PataraiaRumiana Dimova
Jun 27, 2014·Physical Chemistry Chemical Physics : PCCP·Md Zahidul IslamMasahito Yamazaki

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Citations

Feb 2, 2016·Biochimica Et Biophysica Acta·Susana Moreno-Flores
Jun 17, 2015·Biochimica Et Biophysica Acta·Marc-Antoine SaniFrances Separovic
Aug 13, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Delin SunClifford E Woodward
Apr 30, 2016·Physical Chemistry Chemical Physics : PCCP·Mohammad Abu Sayem KaralMasahito Yamazaki
Nov 12, 2016·Applied Microbiology and Biotechnology·Nathaly Marín-MedinaChad Leidy
Feb 7, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Md Zahidul IslamMasahito Yamazaki
Mar 11, 2018·Applied Microbiology and Biotechnology·Md Zahidul IslamMasahito Yamazaki
Mar 29, 2019·European Biophysics Journal : EBJ·Mohammad Abu Sayem KaralMd Mostofa Shakil
Sep 26, 2019·European Biophysics Journal : EBJ·Mohammad Abu Sayem KaralKhondkar Siddique-E-Rabbani
Dec 5, 2019·European Biophysics Journal : EBJ·Mohammad Abu Sayem KaralZaid Bin Mahbub
Jul 2, 2018·The Journal of Chemical Physics·Moynul HasanMasahito Yamazaki
Aug 1, 2019·The Journal of Membrane Biology·Edel Cunill-Semanat, Jesús Salgado
Jun 5, 2020·European Biophysics Journal : EBJ·Md Kabir AhamedShareef Ahammed
Jun 15, 2020·European Biophysics Journal : EBJ·Mohammad Abu Sayem KaralShareef Ahammed
Feb 7, 2020·Nature·Yuko FujiokaNobuo N Noda
Sep 9, 2020·Biochemical Society Transactions·Jahangir Md Alam, Nobuo N Noda
May 31, 2020·Journal of Fluorescence·Mohammad Abu Sayem KaralZaid Bin Mahbub
Feb 20, 2020·Physical Review. E·Mohammad Abu Sayem KaralMd Mostofa Shakil
Dec 8, 2019·Biophysical Journal·Md Mizanur Rahman MoghalMasahito Yamazaki
Nov 27, 2020·Biophysics and Physicobiology·Kazuma Yasuhara, Kenichi Morigaki
Jan 25, 2020·Biochimie·Sathishkumar MunusamyCarlos Munoz-Garay
Jan 31, 2017·Biochimica Et Biophysica Acta. Biomembranes·Nawal K KhadkaJianjun Pan
Aug 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Mohammad Abu Sayem KaralMasahito Yamazaki
Nov 10, 2018·Biochimica Et Biophysica Acta. Biomembranes·Farliza ParvezMasahito Yamazaki
Feb 10, 2018·Chemistry and Physics of Lipids·Md Mizanur Rahman MoghalMasahito Yamazaki
Apr 29, 2021·The European Physical Journal. E, Soft Matter·Marzuk AhmedMuhammad Samir Ullah
May 15, 2021·PloS One·Malay Kumar SarkarSayed Ul Alam Shibly
Jul 16, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Sijia WangHonglai Liu
Jul 10, 2021·Nature Structural & Molecular Biology·Tatsuro MaruyamaNobuo N Noda
Aug 12, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Manami TsukamotoKazuma Yasuhara
May 9, 2019·The Journal of Physical Chemistry. B·Victor LevadnyyMasahito Yamazaki
Feb 16, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Moynul HasanMasahito Yamazaki
Jan 10, 2019·Chemical Reviews·Shantanu GuhaWilliam C Wimley
Jun 17, 2020·The Journal of Physical Chemistry. B·Samiron Kumar SahaMasahito Yamazaki

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