Stretch-activated whole-cell currents in smooth muscle cells from mesenteric resistance artery of guinea-pig

The Journal of Physiology
M SetoguchiM Fujishima

Abstract

1. Stretch-activated (SA) channels were studied in smooth muscle cells isolated from mesenteric resistance arteries using the whole-cell patch-clamp method. Membrane stretch was achieved by cell inflation after application of positive pressure through a patch electrode. 2. In the voltage-clamp configuration, cell inflation increased and cell deflation decreased the membrane conductance. Conductance of the evoked current depended on the increase in cross-sectional area of the cell. The current-voltage relationship was linear between -80 and 0 mV, while further hyperpolarization showed a slight inward rectification. 3. The reversal potential of the SA current depended on the extracellular Na+ concentration, suggesting that the inward SA current was carried predominantly by Na+. The SA current was also carried by other cations, suggesting that the channel responsible for this current is a non-selective cation channel. The permeability sequence of cations as assessed by reversal potential was as follows: K+ > or = CS+ > or = Na+ > Li+. The channel was also permeable to Ca2+. 4. Extracellular Ca2+ and Gd3+ inhibited the SA current carried by monovalent cations in a concentration-dependent manner with IC50 (concentration giving 50% o...Continue Reading

References

Oct 1, 1992·The Journal of Physiology·N HagiwaraH Irisawa
Apr 1, 1992·The American Journal of Physiology·M J DavisJ D Hood
Jan 11, 1991·Pflügers Archiv : European journal of physiology·T HisadaJ V Walsh
Sep 1, 1988·Pflügers Archiv : European journal of physiology·M T KirberJ J Singer
Jul 1, 1985·The American Journal of Physiology·H J BryantF J Haddy
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jan 1, 1993·EXS·X C Yang, F Sachs
Nov 1, 1994·The Journal of Physiology·M C Wellner, G Isenberg
Jun 1, 1994·Hypertension·G D'Angelo, G A Meininger

❮ Previous
Next ❯

Citations

Nov 6, 1998·Pharmacology & Therapeutics·A R Wright, S A Rees
Mar 8, 2002·Brain Research. Developmental Brain Research·Guo Qi TengWilliam J Pearce
Aug 17, 2010·Acta Pharmacologica Sinica·Michael Sze-Ka Wong, Paul Michel Vanhoutte
Jun 9, 2000·Annual Review of Physiology·H Kiriazis, E G Kranias
May 17, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Brian E Carlson, Daniel A Beard
Aug 3, 2011·International Journal of Vascular Medicine·Eric C PetersonGavin Britz
Jan 15, 2005·Journal of Vascular Research·S Wijetunge, A D Hughes
Jan 21, 2000·Hypertension·W F Jackson
Aug 30, 2003·American Journal of Respiratory Cell and Molecular Biology·Yasushi ItoHiroaki Kume
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J S ShamH Cheng
Mar 16, 2000·The Journal of Physiology·Y H ZhangY E Earm
Jul 26, 2011·Journal of Theoretical Biology·Hannah Farr, Tim David
Jun 9, 2009·Pharmacology & Therapeutics·Ryuji InoueYasuhiro Kawarabayashi
Nov 7, 2008·The EMBO Journal·Michael Mederos y SchnitzlerThomas Gudermann
Sep 14, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R G LentleP W M Janssen
Nov 24, 2004·European Journal of Pharmacology·Damon PoburkoUrs T Ruegg
Mar 3, 1999·The Journal of General Physiology·N ShirokovaH Cheng
Feb 2, 2000·The Journal of General Physiology·A GonzálezE Ríos
Feb 1, 2015·Seminars in Cell & Developmental Biology·Lance L Munn
Apr 9, 1998·Biophysical Journal·C SolsonaJ M Fernández
Dec 6, 2008·Journal of Pharmacological Sciences·Toshihide KashiharaTomohisa Ishikawa
Jul 11, 2008·Progress in Biophysics and Molecular Biology·Christelle GuibertJean-Pierre Savineau
Dec 9, 2003·The Journal of Biological Chemistry·Christopher W WardMartin F Schneider
Feb 28, 2002·Circulation Research·Donald G WelshJoseph E Brayden
Sep 27, 2003·Circulation Research·Katsuhiko MurakiYuji Imaizumi
Nov 5, 2004·American Journal of Physiology. Cell Physiology·Shunsuke YanoKoichi Nakayama

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