Stretch-dependent activation and desensitization of mitogen-activated protein kinase in carotid arteries

The American Journal of Physiology
M T FranklinL P Adam

Abstract

Arterial smooth muscle stretch is an important physiological modulator of vascular function. To identify intracellular processes altered during muscle stretch, we found previously that extracellular signal-regulated kinase-mitogen-activated protein kinase (MAPK) activity increased in response to the application of mechanical loads. In the present study, stretch-dependent activation of MAPK in porcine carotid arteries was investigated as was the phosphorylation of the thin filament-binding protein caldesmon, which is known to be a substrate for the kinase in fully differentiated smooth muscle. MAPK activity was 67 pmol.min-1.mg protein-1 in unloaded muscle strips immediately after attachment to force transducers and 139 pmol.min-1.mg protein-1 within 30 s of muscle stretch. When muscle strips were continually stretched, MAPK activity remained elevated for approximately 2 h and then decreased over 16 h to 16 pmol.min-1.mg protein-1. When muscle strips were stretched and then unloaded, MAPK activity decreased within 1 h to the level present in the muscle before the stretch. These effects of muscle stretch on MAPK activity were additive to the effects of KCl or phorbol ester stimulation and were partially inhibited by reducing extr...Continue Reading

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Citations

Feb 9, 2000·American Journal of Physiology. Heart and Circulatory Physiology·B E SpurrellM A Hill
Jun 14, 2000·American Journal of Physiology. Heart and Circulatory Physiology·I A YambolievW T Gerthoffer
Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·T V MurphyM A Hill
Jun 25, 2004·Journal of Pharmacological Sciences·Seongchun KwonHee Yul Ahn
Oct 1, 2004·The Journal of Biological Chemistry·D Brian FosterC-L Albert Wang
Feb 3, 2006·Journal of Biomedical Science·Jolanta KordowskaChih-Lueh Albert Wang
May 1, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sonemany SalinthoneWilliam T Gerthoffer
Jun 6, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Talaibek BorbievJoe G N Garcia
Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M J DavisG A Meininger
Apr 30, 1999·Physiological Reviews·M J Davis, M A Hill
Feb 7, 2001·American Journal of Physiology. Cell Physiology·J C HedgesW T Gerthoffer
Jan 18, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jeremy GoldmanShu Q Liu
Mar 27, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Renan J SandovalWilliam J Pearce
Oct 11, 2005·Neurosurgical Focus·Eiichi Tani
Apr 12, 2013·Biology of Reproduction·Juliana MeolaRui Alberto Ferriani
Jun 13, 2002·Clinical and Experimental Pharmacology & Physiology·Timothy V MurphyMichael A Hill
Dec 11, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·L LoufraniD Henrion
Jan 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Gerard D'Angelo, Leonard P Adam
Jun 14, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Brian E SpurrellMichael A Hill
May 22, 2002·European Journal of Pharmacology·Timothy V MurphyMichael A Hill
Feb 13, 2001·Current Opinion in Genetics & Development·G Pawlak, D M Helfman

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