Stretch-induced Ca2+ independent ATP release in hippocampal astrocytes

The Journal of Physiology
Yingfei XiongZhuan Zhou

Abstract

Similar to neurons, astrocytes actively participate in synaptic transmission via releasing gliotransmitters. The Ca2+ -dependent release of gliotransmitters includes glutamate and ATP. Following an 'on-cell-like' mechanical stimulus to a single astrocyte, Ca2+ independent single, large, non-quantal, ATP release occurs. Astrocytic ATP release is inhibited by either selective antagonist treatment or genetic knockdown of P2X7 receptor channels. Our work suggests that ATP can be released from astrocytes via two independent pathways in hippocampal astrocytes; in addition to the known Ca2+ -dependent vesicular release, larger non-quantal ATP release depends on P2X7 channels following mechanical stretch. Astrocytic ATP release is essential for brain functions such as synaptic long-term potentiation for learning and memory. However, whether and how ATP is released via exocytosis remains hotly debated. All previous studies of non-vesicular ATP release have used indirect assays. By contrast, two recent studies report vesicular ATP release using more direct assays. In the present study, using patch clamped 'ATP-sniffer cells', we re-investigated astrocytic ATP release at single-vesicle resolution in hippocampal astrocytes. Following an 'o...Continue Reading

References

Dec 11, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M WightmanO H Viveros
May 1, 1984·The Biochemical Journal·M JadotR Wattiaux
Feb 7, 1997·Science·E A Newman, K R Zahs
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M L CotrinaM Nedergaard
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·R A North, A Surprenant
Jan 16, 2002·The Journal of Biological Chemistry·Charles E StoutAndrew C Charles
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Emma J Blott, Gillian M Griffiths
Sep 25, 2002·Physiological Reviews·R Alan North
Nov 5, 2002·The Journal of Biological Chemistry·Silvia CocoClaudia Verderio
Apr 11, 2003·Journal of Neurophysiology·Mark DarbyBrian A MacVicar
Dec 17, 2003·Journal of Neurochemistry·Christopher M AndersonRaymond A Swanson
Jan 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiao YuZhuan Zhou
Aug 5, 2004·Journal of Neuropathology and Experimental Neurology·Heike FrankePeter Illes
Jul 19, 2005·Nature Reviews. Neuroscience·Andrea Volterra, Jacopo Meldolesi
Oct 7, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaoke ChenZhuan Zhou
Oct 8, 2005·Science·Olivier PascualPhilip G Haydon
Feb 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sylvia O SuadicaniEliana Scemes
Mar 28, 2006·Trends in Neurosciences·Christopher M Anderson, Maiken Nedergaard
Jul 4, 2006·Physiological Reviews·Philip G Haydon, Giorgio Carmignoto
Jan 9, 2007·Trends in Molecular Medicine·Michael M HalassaPhilip G Haydon
Feb 20, 2007·Cancer Research·Charles P K LaiChristian C Naus
Feb 21, 2007·Nature Neuroscience·Pascal JourdainAndrea Volterra
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·David N Bowser, Baljit S Khakh
Jul 10, 2007·Nature Cell Biology·Zhijun ZhangShumin Duan
Jul 14, 2007·The Journal of Biological Chemistry·Tina PangršičRobert Zorec
Aug 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jyoti K JaiswalSanford M Simon
Oct 30, 2007·Nature Neuroscience·David J RossiClaudia Mohr
May 2, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jian KangMaiken Nedergaard
May 9, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jeremy PetraviczKen D McCarthy
Jul 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dongdong LiMartin Oheim
Oct 15, 2008·The Journal of General Physiology·Zonghe YanStanko S Stojilkovic
Oct 31, 2008·Nature·Grant R J GordonBrian A MacVicar
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Weiguo PengMaiken Nedergaard
Dec 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Dongdong LiNicole Ropert
Jul 22, 2010·Science·Alexander V GourineSergey Kasparov
Jul 22, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tao LiuZhuan Zhou

❮ Previous
Next ❯

Citations

Aug 7, 2018·Frontiers in Molecular Neuroscience·Yingfei XiongZhuan Zhou
Oct 12, 2020·Neuroscience Bulletin·Sasa Jovanovic, Ivan Milenkovic
Jan 18, 2021·Brain : a Journal of Neurology·Cornelius K DonatMazdak Ghajari
Dec 17, 2020·Frontiers in Neurology·Quirijn P VerhoogErwin A van Vliet
Apr 29, 2021·Signal Transduction and Targeted Therapy·Zhao HuangYong Tang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

© 2022 Meta ULC. All rights reserved