Stretch-induced myosin light chain phosphorylation in rat uterus

Archives of Biochemistry and Biophysics
S CsabinaK BARANY

Abstract

Stretching of rat uterine strips induced phosphorylation of the 20,000-Da light chain of myosin to the same extent as was observed in strips contracted by carbachol or oxytocin. Stretching also reversed the partial dephosphorylation of light chain caused by treatment with ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) for 1 min. However, complete dephosphorylation of the light chain with 50-min EGTA-treatment could not be reversed by stretch. When stretched uterine strips containing light chain with a phosphate content greater than 0.75 mol/mol were quick-released, active force developed. On the other hand, when the phosphate content of light chain was reduced to less than 0.25 mol/mol, quick-release of the stretched strips did not produce active force. It is shown that Ca2+ mobilized from intracellular sources is involved in stretch-induced phosphorylation. The data indicate that myosin light chain phosphorylation is a prerequisite for active force development in smooth muscle.

References

Jun 23, 1986·Biochimica Et Biophysica Acta·S CsabinaK Bárány
Jan 1, 1985·Annual Review of Pharmacology and Toxicology·K E Kamm, J T Stull
Jan 1, 1983·Progress in Biophysics and Molecular Biology·S B Marston
Sep 1, 1983·Archives of Biochemistry and Biophysics·K BárányM Bárány
Jan 1, 1984·General Pharmacology·O Arjamaa
Sep 16, 1980·Biochemical and Biophysical Research Communications·R A JanisR T Gualtieri
May 28, 1902·The Journal of Physiology·W M Bayliss

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Citations

Feb 25, 1987·Biochimica Et Biophysica Acta·K BárányM Bárány
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S CsabinaK Bárány
Apr 23, 2005·American Journal of Obstetrics and Gynecology·Bryan F MitchellSandra T Davidge
Jan 1, 1993·The American Journal of Physiology·S Wray

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