Abstract
The advent of single-molecule biology has allowed unprecedented insight into the dynamic behavior of biological macromolecules and their complexes. Unexpected properties, masked by the asynchronous behavior of myriads of molecules in bulk experiments, can be revealed; equally importantly, individual members of a molecular population often exhibit distinct features in their properties. Finally, the single-molecule approaches allow us to study the behavior of biological macromolecules under applied tension or torsion: understanding the mechanical properties of these molecules helps us understand how they function in the cell. The aim of this chapter is to summarize and critically evaluate the properties of single DNA molecules and of single chromatin fibers. The use of the high-resolution imaging capabilities of the atomic force microscopy has been covered, together with manipulating techniques such as optical fibers, optical and magnetic tweezers, and flow fields. We have learned a lot about DNA and how it responds to applied forces. It is also clear that even though the study of the properties of individual chromatin fibers has just begun, the single-molecule approaches are expected to provide a wealth of information concerning...Continue Reading
Citations
May 6, 2003·The Journal of Biological Chemistry·Jordanka Zlatanova
Apr 6, 2007·Journal of Biomechanical Engineering·Mallika SridharMichael F Insana
Aug 19, 2009·Optics Express·Yen-Heng LinGwo-Bin Lee
May 15, 2015·Journal of Cardiovascular Translational Research·Ares Pasipoularides
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jordanka Zlatanova, Sanford H Leuba
Mar 18, 2005·The Journal of Biological Chemistry·Cyril ClaudetJan Bednar
Aug 27, 2005·Journal of Biomechanical Engineering·Ronald P JeanAlexander A Spector
Feb 4, 2010·Cardiovascular Research·Allen KaasikVladimir Veksler
May 8, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sanford H LeubaSaleem A Khan
Jun 7, 2008·Circulation Research·Kris Noel DahlJan Lammerding
Dec 9, 2014·Nucleus·Paula A Vasquez, Kerry Bloom
Jun 22, 2010·Molecular & Cellular Proteomics : MCP·Marian BaclayonGijs J L Wuite
May 19, 2010·International Journal of Molecular Sciences·Christophe LavelleJordanka Zlatanova
Dec 29, 2004·Biophysical Chemistry·Claudio AnselmiAnita Scipioni
Jun 15, 2012·Bioconjugate Chemistry·Shige H YoshimuraKunio Takeyasu
Dec 18, 2013·ACS Nano·Michal Levy-SakinYuval Ebenstein
Jun 28, 2019·ACS Nano·Maged F SeragSatoshi Habuchi
Nov 4, 2006·Molecular Cell·Jordanka Zlatanova, Kensal van Holde