PMID: 9169862May 15, 1997Paper

Striatal target-induced axonal branching of dopaminergic mesencephalic neurons in culture via diffusible factors

Journal of Neuroscience Research
M ManierC Feuerstein

Abstract

The effects of striatal target cells on the morphological development of dopaminergic neurons were studied in dissociated cultures of embryonic rat mesencephalon. Mesencephalic neurons were cultured for four days in presence of target striatal cells or non target cerebellar ones. The outgrowth of dopaminergic neurons, visualized after tyrosine hydroxylase immunohistochemistry, was examined by quantitative morphometry. In cocultures, the increased complexity of dopaminergic neurites (branching) was the most striking pattern. It was dependent on the presence of target striatal cells as compared to non target ones. Cultures raised in presence or absence of serum lead to suggest the implication of striatal neurons rather than glia. Using MAP2 and phosphorylated neurofilaments immunohistochemistry in combination with tyrosine hydroxylase immunolabelling, it could be shown that the target-induced branching effect concerned only axonal and not dendritic processes. To further define whether diffusible factors from the striatal target would participate in the axonal branching effect, mesencephalic cells were cultured in conditioned medium from striatal neurons. Striatal conditioned medium enhanced dopamine uptake and dopamine neuron bra...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A ProchiantzJ Glowinski
Jul 1, 1991·Trends in Neurosciences·M Tessier-Lavigne, M Placzek
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B KnüselF Hefti
Jan 1, 1990·Developmental Biology·A RousseletA Prochiantz
Sep 1, 1990·Brain Research Bulletin·M MorissetteT Di Paolo
Jun 1, 1989·Brain Research Bulletin·C JaegerR Llinás
Mar 1, 1986·Brain Research Bulletin·P MouchetJ Thibault
Apr 15, 1981·Biochemical and Biophysical Research Communications·J ThibaultF Gros
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L M HemmendingerA Heller
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·K Herrmann, C J Shatz
Oct 1, 1995·Neuron·A Prochiantz
Sep 1, 1993·Developmental Biology·S A BermanS Bursztajn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amygdala and Midbrain Dopamine

The midbrain dopamine system is widely studied for its involvement in emotional and motivational behavior. Some of these neurons receive information from the amygdala and project throughout the cortex. When the circuit and transmission of dopamine is disrupted symptoms may present. Here is the latest research on the amygdala and midbrain dopamine.