Strikingly different localization of galectin-3 and galectin-4 in human colon adenocarcinoma T84 cells. Galectin-4 is localized at sites of cell adhesion.

The Journal of Biological Chemistry
Margaret E HuflejtHakon Leffler

Abstract

Two beta-galactoside-binding proteins were found to be prominently expressed in the human colon adenocarcinoma T84 cell line. Cloning and sequencing of one, a 36-kDa protein, identified it as the human homolog of galectin-4, a protein containing two carbohydrate binding domains and previously found only in the epithelial cells of the rat and porcine alimentary tract. The other, a 29-kDa protein, is galectin-3, containing a single carbohydrate binding domain, previously found in a number of different cell types including human intestinal epithelium. Despite the marked similarities in the carbohydrate binding domains of these two galectins, their cellular distribution patterns are strikingly different and vary with cellular conditions. In confluent T84 cells, galectin-4 is mostly cytosolic and concentrated at the basal membrane, whereas galectin-3 tends to be concentrated in large granular inclusions mostly at the apical membrane. In subconfluent T84 cells, each galectin is distributed to specific domains of lamellipodia, with galectin-4 concentrated in the leading edge and galectin-3 more proximally. Such different localization of galectins-4 and -3 within T84 cells implies different targeting mechanisms, ligands, and functions....Continue Reading

References

May 1, 1992·Biochemical Society Transactions·J L WangR J Patterson
Dec 1, 1992·The Journal of Cell Biology·M L ChiuE J O'Keefe
Jan 1, 1990·Methods in Enzymology·K Dharmsathaphorn, J L Madara
Dec 1, 1989·The Journal of Experimental Medicine·B J CherayilS Pillai
Nov 14, 1989·Biochemistry·H LefflerS H Barondes
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I K MoutsatsosJ L Wang
Mar 1, 1984·The American Journal of Physiology·S GrinsteinE W Gelfand
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D R McClayR B Marchase
May 5, 1995·The Journal of Biological Chemistry·S P ColganJ L Madara
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M M LotzA M Mercurio
Mar 1, 1995·Glycobiology·Y OzekiK Titani
Mar 10, 1995·The Journal of Biological Chemistry·M A GittS H Barondes
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·R Y YangF T Liu

❮ Previous
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Citations

May 5, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C ColnotF Poirier
Sep 3, 2010·Inflammatory Bowel Diseases·Manjula PuthenedamJohn H Kwon
Aug 28, 2001·Biochimie·R C Hughes
May 27, 2003·Biochimie·Joelle BottiChristian Derappe
Sep 12, 2002·Biochimica Et Biophysica Acta·Fu-Tong LiuJohn L Wang
Dec 2, 1999·Biochimica Et Biophysica Acta·R C Hughes
Mar 24, 2005·Thyroid : Official Journal of the American Thyroid Association·L A T ArnaldiJ M Cerutti
Jul 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Veronika KrejciríkováJirí Brynda
Mar 26, 2013·Applied and Environmental Microbiology·Devon KavanaughRita M Hickey
Sep 18, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Junko Nio-KobayashiToshihiko Iwanaga
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ola BlixtJames C Paulson
Feb 18, 2016·BioMed Research International·Mattias BlockMichael E Breimer
Aug 6, 2011·European Journal of Pharmacology·Sander de KivitLinette E M Willemsen
Aug 5, 2009·Traffic·Delphine DelacourRalf Jacob
Mar 13, 2008·The Journal of Pathology·P DemetterI Salmon
Mar 21, 2012·Glia·Mirjana StancicWia Baron
Apr 27, 2011·International Journal of Cancer. Journal International Du Cancer·Hannah BarrowLu-Gang Yu
Mar 12, 2005·Biochimie·Melissa A WootersAlan K Erickson
Mar 28, 2016·Protein & Cell·Zhan-Qi Cao, Xiu-Li Guo
Dec 25, 2007·The American Journal of Pathology·Maurice NachtigalEugene P Mayer
Oct 12, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·F T Liu
Aug 26, 2016·Immunological Reviews·Jennifer C Brazil, Charles A Parkos
Sep 20, 2016·Scientific Reports·Joane K RustiguelMaria Cristina Nonato
Jan 28, 2017·Developmental and Comparative Immunology·Abirami ArasuJesu Arockiaraj
Jan 31, 2013·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Denis HouzelsteinDominique Higuet
Jun 16, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Junko NioToshihiko Iwanaga
Dec 19, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K Wasano, Y Hirakawa
Mar 7, 2006·Journal of Cellular Biochemistry·Tricia A UlmerSuzanne Laferté
Sep 5, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·D CaseS Majka
Oct 3, 2014·Journal of Glaucoma·Margaret E HuflejtMarko I Vuskovic
Jul 4, 2001·The Journal of Biological Chemistry·K HottaY Matsuzawa
Jan 24, 2014·International Journal of Oncology·Maria C VladoiuYves St-Pierre
Jun 4, 1998·International Journal of Cancer. Journal International Du Cancer·M J ScanlanL J Old
Feb 28, 1998·The Journal of Biological Chemistry·M A GittH Leffler

❮ Previous
Next ❯

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