Stringent response interacts with the ToxR regulon to regulate Vibrio cholerae virulence factor expression.

Archives of Microbiology
David M RaskinZachary Lundy

Abstract

The epidemic diarrheal disease cholera is caused by the Gram-negative bacterium Vibrio cholerae. V. cholerae virulence factors include the toxin-coregulated pilus (TCP) and cholera toxin, which are major factors responsible for host colonization and production of diarrhea. Expression of cholera toxin and TCP genes is controlled by the ToxR regulon. The ToxR regulon includes the transcriptional activators ToxR, TcpP, and ToxT. ToxT directly initiates transcription of the cholera toxin and TCP genes. TcpP and ToxR are necessary for expression of toxT. TcpP and ToxR activity requires TcpH and ToxS, respectively. Additionally, ToxR is able to directly initiate transcription of the cholera toxin genes independent of TcpP and ToxT. TCP is required early in infection to colonize the small intestine, then cholera toxin is expressed later in infection to produce diarrhea. We tested whether stringent response, the low nutrient stress response, was involved in regulation of virulence genes. Using an infant mouse model, we found that V. cholerae strains with deletions of the stringent response genes were unable to colonize the small intestine. We further tested these stringent response-null mutants and found that stringent response was nec...Continue Reading

References

Sep 1, 1985·Journal of Clinical Microbiology·M Iwanaga, K Yamamoto
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·V L Miller, J J Mekalanos
May 1, 1995·Molecular Microbiology·R C Brown, R K Taylor
Jan 1, 1994·Methods in Enzymology·C L Gardel, J J Mekalanos
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·V J DiRitaP M Bruss
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·C C Häse, J J Mekalanos
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·James BinaJohn Mekalanos
Oct 11, 2003·Developmental Cell·Jun Zhu, John J Mekalanos
Mar 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Susan M Butler, Andrew Camilli
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Deborah T Hung, John J Mekalanos
Oct 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jyl S Matson, Victor J DiRita
Nov 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carsten MatzStaffan Kjelleberg
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·David M RaskinJohn J Mekalanos
Sep 19, 2007·Infection and Immunity·Jyl S MatsonVictor J DiRita
Oct 31, 2007·Archives of Microbiology·Bhabatosh Das, Rupak K Bhadra
May 6, 2008·Annual Review of Microbiology·Katarzyna Potrykus, Michael Cashel
Jul 1, 2009·Infection and Immunity·Basel H Abuaita, Jeffrey H Withey
Jun 3, 2010·Infection and Immunity·Rita TamayoAndrew Camilli
Jul 6, 2010·Journal of Bacteriology·Sanjat KanjilalStephen B Calderwood
Feb 18, 2012·Nature Reviews. Microbiology·Zachary D Dalebroux, Michele S Swanson
Apr 3, 2012·Journal of Bacteriology·Huajun HeDavid M Raskin
Jul 21, 2012·Molecular Microbiology·Usheer KanjeeWalid A Houry
Apr 1, 2015·Infection and Immunity·Nicole AcostaTracy L Raivio
Sep 1, 2015·Molecular Microbiology·Salvador Almagro-MorenoRonald K Taylor
May 6, 2016·Molecular Microbiology·Wieland Steinchen, Gert Bange
May 27, 2016·Microbiology Spectrum·Jenna G ConnerFitnat H Yildiz
Oct 12, 2016·Microbiology Spectrum·Donatella LippiSaverio Caini
Mar 18, 2017·Lancet·John D ClemensJan Holmgren
Jan 10, 2018·Pathogens and Disease·Kenneth M Peterson, Patrick S Gellings
Sep 12, 2018·Annual Review of Microbiology·Richard L GourseWilma Ross
Nov 27, 2018·Frontiers in Microbiology·Matthew J Dorman, Charles J Dorman
Jan 9, 2019·Infection and Immunity·Mondraya F HowardJames E Bina

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Citations

Jun 17, 2020·Environmental Microbiology Reports·Jane M JayakumarSalvador Almagro-Moreno
Oct 31, 2020·Frontiers in Cellular and Infection Microbiology·Ashok KumarNiraj Kumar
Apr 13, 2021·ACS Infectious Diseases·Denise ChacAna A Weil

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Methods Mentioned

BETA
PCR
reverse-transcription PCR
enzyme-linked immunosorbent assays
ELISA
motility assay

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