Stringent response of Bacillus stearothermophilus: evidence for the existence of two distinct guanosine 3',5'-polyphosphate synthetases.

Journal of Bacteriology
S Fehr, D Richter

Abstract

Bacillus stearothermophilus reacted to pseudomonic acid-induced inhibition of isoleucine-transfer ribonucleic acid (RNA) acylation and to energy downshift caused by alpha-methylglucoside addition with accumulation of guanosine 3',5'-polyphosphates [(p)ppGpp] and restriction of RNA synthesis. In vitro studies indicated that (p)ppGpp was synthesized by two different enzymes. One enzyme, (p)ppGpp synthetase I, was present in the ribosomal fraction, required the addition of a ribosome-messenger RNA-transfer RNA complex for activation, and was inhibited by tetracycline and thiostrepton. It is suggested that (p)ppGpp synthetase I is comparable to the relA gene product from Escherichia coli and is responsible for (p)ppGpp accumulation during amino acid starvation. The other enzyme, (p)ppGpp synthetase II, was found in the high-speed supernatant fraction (S100). It functioned independently of ribosomes, transfer RNA, and messenger RNA and was not inhibited by the above-mentioned antibiotics. (p)ppGpp synthetase II is thought to be responsible for (p)ppGpp accumulation during carbon source downshift. The two enzymes differ in their Km values for adenosine triphosphate (ATP):2mM ATP for synthetase I and 0.05 mM ATP for synthetase II. The...Continue Reading

References

Jan 1, 1979·Annual Review of Genetics·J A Gallant
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E A Heinemeyer, D Richter
Jan 1, 1977·Annual Review of Genetics·M Nomura, E A Morgan
Dec 1, 1975·The Biochemical Journal·A OgilvieW Kersten
Oct 1, 1975·Journal of Bacteriology·J J RoweR E Amelunxen
Mar 25, 1963·Biochemical and Biophysical Research Communications·D P KESSLER, H V RICKENBERG

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Citations

Aug 1, 1988·Molecular & General Genetics : MGG·E BaracchiniH Bremer
Jan 1, 1983·Zeitschrift für allgemeine Mikrobiologie·K Riedel
Oct 18, 2000·FEMS Microbiology Letters·T M WendrichM A Marahiel
Aug 10, 2000·Molecular Microbiology·M MacvaninD Hughes
Mar 1, 1984·Biotechnology and Bioengineering·M M DomachM L Shuler
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Patrick P DennisHans Bremer
May 3, 1996·The Journal of Biological Chemistry·O H Martínez-CostaF Malpartida
Dec 1, 1995·Microbiological Reviews·C CondonC L Squires
Mar 1, 1996·Journal of Bacteriology·U MecholdH Malke

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