Stripping voltammetric detection of insulin at liquid-liquid microinterfaces in the presence of bovine albumin

The Analyst
Shane O'SullivanDamien W M Arrigan


Electrochemistry at the interface between two immiscible electrolyte solutions (ITIES) provides a platform for label-free detection of biomolecules. In this study, adsorptive stripping voltammetry (AdSV) was implemented at an array of microscale ITIES for the detection of the antidiabetic hormone insulin. By exploiting the potential-controlled adsorption of insulin at the ITIES, insulin was detected at 10 nM via subsequent voltammetric desorption. This is the lowest detected concentration reported to-date for a protein by electrochemistry at the ITIES. Surface coverage calculations indicate that between 0.1 and 1 monolayer of insulin forms at the interface over the 10-1000 nM concentration range of the hormone. In a step toward assessment of selectivity, the optimum adsorption potentials for insulin and albumin were determined to be 0.900 V and 0.975 V, respectively. When present in an aqueous mixture with albumin, insulin was detected by tuning the adsorption potential to 0.9 V, albeit with reduced sensitivity. This provides the first example of selective detection of one protein in the presence of another by exploiting optimal adsorption potentials. The results presented here provide a route to the improvement of detection li...Continue Reading


Mar 1, 1987·Diabetes·E Helmerhorst, G B Stokes
Sep 1, 2005·Analytical Chemistry·Jidong GuoShigeru Amemiya
Jun 27, 2007·The Analyst·Grégoire Herzog, Damien W M Arrigan
Dec 25, 2007·Chemical Reviews·Joseph Wang
Jan 29, 2008·Colloids and Surfaces. B, Biointerfaces·Anne Engelbrecht ThomsenJesper Ostergaard
May 10, 2008·Chemical Reviews·Adam Heller, Ben Feldman
Jun 26, 2008·Analytical Chemistry·Micheál D ScanlonDamien W M Arrigan
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Francine KivlehanDamien W M Arrigan
Feb 23, 2010·Trends in Analytical Chemistry : TRAC·Jerzy Silberring, Pawel Ciborowski
Jun 11, 2010·Physical Chemistry Chemical Physics : PCCP·Micheál D ScanlonDamien W M Arrigan
Aug 6, 2010·Analytical and Bioanalytical Chemistry·Shigeru AmemiyaBenjamin Kabagambe
Dec 3, 2010·Chemical Society Reviews·Xin HuangJiacong Shen
Mar 11, 2011·Chemical Society Reviews·Shujuan LiuYuanhua Shao
Mar 31, 2011·Electrophoresis·Haleem J IssaqTimothy D Veenstra
Jul 31, 2012·Biosensors & Bioelectronics·Mengyun XuJason J Davis
Aug 1, 2012·Analytical and Bioanalytical Chemistry·Mian YangAlexandra Ros
Aug 3, 2012·Journal of Nanoscience and Nanotechnology·Jeung Hee AnJeong-Woo Choi
Dec 15, 2012·Analytical and Bioanalytical Chemistry·Eva Alvarez de EulateDamien W M Arrigan
Mar 19, 2013·Analytica Chimica Acta·Grégoire Herzog, Valerio Beni
Jan 1, 2013·Biosensors·Jon Stefan Hansen, Jørn Bolstad Christensen

Related Concepts

Bos indicus
Serum Albumin, Bovine
Liquid Phase Microextraction

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Related Papers

Analytical and Bioanalytical Chemistry
Eva Alvarez de EulateDamien W M Arrigan
Chemical Communications : Chem Comm
Damien W M ArriganValerio Beni
Langmuir : the ACS Journal of Surfaces and Colloids
Francine KivlehanDamien W M Arrigan
Langmuir : the ACS Journal of Surfaces and Colloids
Manuel Alejandro MéndezHubert H Girault
© 2021 Meta ULC. All rights reserved