Stroma derived COL6A3 is a potential prognosis marker of colorectal carcinoma revealed by quantitative proteomics

Oncotarget
Jie QiaoFeng Liu

Abstract

Colorectal cancer (CRC) represents the third most common cancer in males and second in females worldwide. Here, we performed a quantitative 8-plex iTRAQ proteomics analysis of the secreted proteins from five colonic fibroblast cultures and three colon cancer epithelial cell lines. We identified 1114 proteins at 0% FDR, including 587 potential secreted proteins. We further recognized 116 fibroblast-enriched proteins which were significantly associated with cell movement, angiogenesis, proliferation and wound healing, and 44 epithelial cell-enriched proteins. By interrogation of Oncomine database, we found that 20 and 8 fibroblast-enriched proteins were up- and downregulated in CRC, respectively. Western blots confirmed the fibroblast-specific secretion of filamin C, COL6A3, COL4A1 and spondin-2. Upregulated mRNA and stroma expression of COL6A3 in CRC, which were revealed by Oncomine analyses and tissue-microarray-immunohistochemistry, indicated poor prognosis. COL6A3 expression was significantly associated with Dukes stage, T stage, stage, recurrence and smoking status. Circulating plasma COL6A3 in CRC patients was upregulated significantly comparing with healthy peoples. Receiver operating characteristic curve analysis revealed...Continue Reading

References

Aug 30, 2005·Biochimica Et Biophysica Acta·Ryoichi YamajiYoshihisa Nakano
Jul 21, 2007·International Journal of Cancer. Journal International Du Cancer·Dong Hyuk KiSun Young Rha
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
May 15, 2009·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Flora AngeliJohn G Delinassios
Jan 16, 2010·The Journal of Pathology·Jun-Mei HaoJian-Ming Li
Oct 13, 2010·The Journal of Experimental Medicine·Yingjie XuMoulay A Alaoui-Jamali
Jan 1, 2007·Proteomics. Clinical Applications·Hanna C DiehlIrmgard Schwarte-Waldhoff
Apr 1, 2009·Proteomics. Clinical Applications·Hong JiRichard J Simpson
May 10, 2011·Annual Review of Biochemistry·Jürgen Cox, Matthias Mann
Oct 13, 2011·Nucleic Acids Research·Suresh MathivananRichard J Simpson
Jun 8, 2013·Journal of Cellular Biochemistry·Esther ImperliniStefania Orrù
Sep 5, 2013·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Christopher Y KangHwyda A Arafat
Sep 13, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sofia TorresJ Ignacio Casal
Jul 29, 2015·Data in Brief·Sun-Xia ChenFeng Liu

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Citations

Dec 5, 2019·International Journal of Molecular Sciences·Mercedes HerreraCristina Peña
Aug 5, 2020·Journal of Experimental & Clinical Cancer Research : CR·Ting WangYujun Hao
Aug 25, 2017·Cancer Science·Makito MiyakeKiyohide Fujimoto
Oct 13, 2020·Frontiers in Cell and Developmental Biology·Claudia Tanja Mierke
Jun 30, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Bérengère Dadone-MontaudiéDaniel Pissaloux
Nov 6, 2020·Movement Disorders : Official Journal of the Movement Disorder Society·Alba Tristán-NogueroÀngels García-Cazorla
Nov 2, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marie-France PenetZaver M Bhujwalla
Dec 3, 2020·International Journal of Molecular Sciences·Cláudia BessaVânia Gonçalves

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Methods Mentioned

BETA
ELISA
gene array
xenograft

Software Mentioned

PASW Statistics
Image
house PERL
GraphPad Prism
Cluster
ProScores
ProteomicS Performance Evaluation Pipeline ( PSPEP
Phobius
TreeView
SecretomeP

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