Stromal C-type lectin receptor COLEC12 integrates H. pylori, PGE2-EP2/4 axis and innate immunity in gastric diseases

Scientific Reports
Lin-Li ChangShau-Ku Huang

Abstract

Tissue stroma is known to be important in regulating Hp-mediated inflammation, but its interaction with Hp and dendritic cells (DCs) remains to be determined. To this end, the potential crosstalk between H. pylori (Hp) infected gastric stromal cells (Hp-GSCs) and DCs was investigated. Primary GSCs from cancerous and adjacent normal tissues were generated from gastric cancer patients, and monocyte-derived DCs were obtained from healthy individuals. Levels of cytokines and prostaglandin E2 (PGE2) were measured by ELISA, and C-type lectin expression in GSCs was assessed by flow cytometry and immunohistochemistry. In a trans-well co-culture system, significantly upregulated DC-derived IL-23 expression was found when DCs were co-cultured with Hp-infected GSCs (Hp-GSCs). Further, PGE2 from Hp-GSCs was discovered to possess the priming effect, which could be inhibited by anti-COLEC12 (Collectin subfamily member 12) Abs, COLEC12 knockdown or when alpha3-fucosyltransferase-null (futB; HP0651) strain of Hp was used. Also, the expression of COLEC12 was co-localized with CD90+ stromal cells in cancerous tissues. Hp-GSCs-conditioned DCs were able to induce the expression of IL-17 from CD4+ T cells, which could be inhibited by IL-23-neutrali...Continue Reading

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Citations

Aug 22, 2020·Journal of Clinical Laboratory Analysis·Guang-Zhang LiZhi-Xin Liu
Sep 15, 2020·World Journal of Gastroenterology : WJG·Rasha Raheem AlkarkoushiTraci L Testerman
Nov 17, 2020·Frontiers in Veterinary Science·Eduardo CasasJohn D Lippolis

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
biopsies
biopsy
ELISA
gene knockdown
Flow cytometry
transfection

Software Mentioned

SPSS
CellQuest

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