Stromal cell-derived factor 1alpha increases polyploidization of megakaryocytes generated by human hematopoietic progenitor cells.

Blood
R GuerrieroH J Hassan

Abstract

The alpha chemokine receptor CXCR4 has been shown to be expressed on human hematopoietic progenitor cells and during the megakaryocytic differentiation pathway. Stromal cell-derived factor 1 (SDF-1) is the ligand for CXCR4. In this study, the role of SDF-1alpha in megakaryocytopoiesis was investigated. CD34(+) progenitors purified from peripheral blood were grown in serum-free liquid suspension culture supplemented with thrombopoietin to obtain a virtually pure megakaryocytic progeny. In this condition, the addition of SDF-1alpha gives rise to megakaryocytes (MKs) showing an increased DNA content and a rise of lobated nuclei, as compared with untreated cells: at day 5, approximately 20% of the cells already showed the presence of more than one nuclear lobe versus fewer than 5% in the control cells; at day 12, approximately 85% of the cells were of large size and markedly polyploid, whereas approximately 60% of the control cells were polyploid, showed fewer lobes, and were a smaller size. This effect was dose-dependent and did not affect the megakaryocytic proliferation. Experiments with the mitogen-activated protein kinase (MAPK) inhibitor PD98059 suggested a role for MAPK pathway on SDF-1alpha-induced endomitosis. Furthermore,...Continue Reading

References

Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·T NagasawaT Kishimoto
Nov 1, 1996·Nature Medicine·B A Premack, T J Schall
Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Nov 5, 1997·The Journal of Biological Chemistry·X SanchezJ Navarro
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·K KawabataT Nagasawa

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Citations

Jul 6, 2012·Annals of Hematology·Stephan EmmrichJan-Henning Klusmann
Jun 24, 2008·Nature Cell Biology·Catherine LabbayeCesare Peschle
Feb 1, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masaaki OdaYuka Nagata
Oct 24, 2001·Journal of Hematotherapy & Stem Cell Research·A OdaK Sawada
Mar 29, 2014·Experimental Hematology·Eun-ju LeeDavid Haylock
Feb 5, 2011·Experimental Hematology·Shannon KiddKent W Christopherson
Dec 21, 2007·Critical Reviews in Oncology/hematology·Cuiling ZhengMin Lu
Jun 13, 2015·Thrombosis and Haemostasis·Darin SiripinSurapol Issaragrisil
Apr 14, 2005·Journal of Cellular Physiology·Elisabetta MelloniGiorgio Zauli
Feb 28, 2003·Clinical and Experimental Immunology·D GibelliniG Zauli
Sep 9, 2006·Stem Cells·Takuya MatsunagaYoshiro Niitsu
Sep 14, 2011·British Journal of Haematology·Huiyuan LiRenchi Yang
Oct 31, 2009·Journal of Thrombosis and Haemostasis : JTH·S SéverinA Mazharian
Jun 2, 2006·Experimental Cell Research·Francesca VulcanoHamisa J Hassan
Jun 7, 2014·British Journal of Clinical Pharmacology·Isabella MassimiFabio M Pulcinelli
Jul 8, 2015·Journal of Thrombosis and Haemostasis : JTH·P Karagiannis, K Eto
Jun 9, 2009·Trends in Biotechnology·Nicholas E Timmins, Lars K Nielsen
Apr 5, 2013·PloS One·Po-Kong ChenDer-Shan Sun
Jan 29, 2011·Journal of Dental Research·Q XingY Ren
Jun 28, 2017·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Hadi RezaeeyanNajmaldin Saki
Aug 22, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Florian H SeegerStefanie Dimmeler
Sep 24, 2019·Platelets·William Vainchenker, Hana Raslova
Oct 26, 2018·Platelets·Sanjeev RaghuwanshiRavi Kumar Gutti
Apr 17, 2014·Journal of Thrombosis and Haemostasis : JTH·F Ferrer-MarinM Sola-Visner
Oct 9, 2021·Journal of Thrombosis and Haemostasis : JTH·Alyssa J Moroi, Peter J Newman

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