Abstract
Osteonecrosis of the femoral head (ONFH) is a devastating orthopedic disease. Previous studies suggested that stromal-cell-derived factor (SDF)-1 was involved in osteogenesis and angiogenesis. However, whether SDF-1 potentiates the angiogenesis and osteogenesis of bone marrow-derived stromal stem cells (BMSCs) in ONFH is not clear. BMSCs were transfected with green fluorescent protein (GFP) or the fusion gene encoding GFP and SDF-1α, and transgenic efficacy was monitored by immunofluorescence. The expression of SDF-1α, runt-related transcription factor 2 (Runx2, osteocalcin (OCN), and alkaline phosphatase (ALP) at the mRNA level was measured by real-time polymerase chain reactions (RT-PCR). The expression of SDF-1α, Runx2, OCN, and p-Smad1/5 were measured at the protein level by Western blot. Transwell migration assay and tube formation assay were utilized to detect the angiogenesis in vitro, whereas the in vivo angiogenesis was monitored by angiography. Immunohistological staining and micro-CT scanning were conducted to assess the histological changes in morphology. In vitro, SDF-1α overexpression in BMSCs promoted osteogenic differentiation and upregulated the expression of osteogenic-related proteins, such as ALP, Runx2, OCN...Continue Reading
Citations
Jun 18, 2019·Journal of Cellular Physiology·Guangjie LiShuanke Wang
Apr 10, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hai-Jia XuZhang-Hua Li
Sep 11, 2019·JBJS Reviews·Masahiro MaruyamaStuart B Goodman
Jul 16, 2019·Journal of Cellular and Molecular Medicine·Yuan LiLei Yang
Nov 7, 2019·International Journal of Molecular Sciences·Tomas KoltaiStephan J Reshkin
Oct 31, 2019·Biomedical Materials·Xiaolin YuFeilong Deng
Sep 9, 2019·Applied Biochemistry and Biotechnology·Valeriya V SolovyevaAlbert A Rizvanov
Mar 22, 2020·Journal of Cellular and Molecular Medicine·Guangjie LiShuanke Wang
Nov 5, 2019·Current Stem Cell Research & Therapy·L Vinod Kumar ReddyDwaipayan Sen
Apr 11, 2020·Journal of Biomedical Materials Research. Part a·Yang LiuLujie Song
Sep 12, 2019·Journal of Cellular and Molecular Medicine·Huachen YuQidong Zhang
Jun 14, 2020·Journal of Bone and Mineral Metabolism·Bo-Hao YinJing-Wen Liu
May 20, 2020·Frontiers in Psychiatry·Yongbo ZhangZheng Zachory Wei
Sep 24, 2020·Stem Cell Research & Therapy·Siddharth ShanbhagKamal Mustafa
Feb 6, 2020·Human Gene Therapy·Peng ShuShu Nan Ye
Aug 17, 2020·Journal of Orthopaedic Surgery and Research·Jing YangXiao Yuan
Nov 24, 2020·Journal of Inflammation Research·Stuart B Goodman, Masahiro Maruyama
Dec 11, 2020·Stem Cells International·Tiago O RibeiroVitor Fortuna
Feb 18, 2020·Pharmacology & Therapeutics·Shiying ShaoYong Chen
Feb 26, 2021·Mechanisms of Ageing and Development·Dhara PatelSadanand Fulzele
May 4, 2021·Frontiers in Cell and Developmental Biology·Feng WangQinglin Kang
Aug 7, 2021·Micromachines·Yun-Liang ChangFeng-Huei Lin