Stromal heterogeneity in pancreatic cancer and chronic pancreatitis.

Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
Lena HaeberleIrene Esposito

Abstract

An abundant stromal reaction is a hallmark of pancreatic ductal adenocarcinoma (PDAC) and chronic pancreatitis (CP). The cells mainly responsible for the stromal reaction are activated pancreatic stellate cells (PSCs). Despite their crucial role, PSCs are not well characterized. PSCs share characteristics with the better-known hepatic stellate cells (HSCs). The aim of this study was a detailed analysis of PSCs in PDAC and CP. Whole-slide specimens of CP (n = 12) and PDAC (n = 10) were studied by histochemistry and immunohistochemistry. The stroma was evaluated using Movat's pentachrome stain. PSCs were tested by immunohistochemistry for PSC markers (α-SMA, CD34, desmin, NGFR, SPARC and tenascin C) and HSC markers (α-crystallin B, CD56, NGF, NT-3, synaptophysin and TrkC). Alpha-SMA, tenascin C, SPARC and NT-3 staining were verified on tissue micro arrays (TMAs) from a well-characterized cohort of 223 PDAC patients. PSCs isolated from human PDAC and CP tissue samples as well as HSCs were evaluated by immunofluorescence. While the stroma of CP cases was characterized by a collagen-rich fibrosis, PDAC stroma displayed higher mucin content (p = 0.0002). PSCs showed variable expression of tested markers. In PDAC samples, staining of ...Continue Reading

Citations

Oct 30, 2019·Journal of Mass Spectrometry : JMS·Jillian JohnsonLingjun Li
Nov 13, 2018·World Journal of Gastroenterology : WJG·Michael Friberg Bruun NielsenSönke Detlefsen
Nov 18, 2020·Cells·Paloma E GarciaMarina Pasca di Magliano
Apr 13, 2021·Frontiers in Pharmacology·Verena HofschröerAlbrecht Schwab

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