Stromal vapors for real-time molecular guidance of breast-conserving surgery.

Scientific Reports
Pierre-Maxence VaysseTiffany Porta Siegel

Abstract

Achieving radical tumor resection while preserving disease-free tissue during breast-conserving surgery (BCS) remains a challenge. Here, mass spectrometry technologies were used to discriminate stromal tissues reported to be altered surrounding breast tumors, and build tissue classifiers ex vivo. Additionally, we employed the approach for in vivo and real-time classification of breast pathology based on electrosurgical vapors. Breast-resected samples were obtained from patients undergoing surgery at MUMC+. The specimens were subsequently sampled ex vivo to generate electrosurgical vapors analyzed by rapid evaporative ionization mass spectrometry (REIMS). Tissues were processed for histopathology to assign tissue components to the mass spectral profiles. We collected a total of 689 ex vivo REIMS profiles from 72 patients which were analyzed using multivariate statistical analysis (principal component analysis-linear discriminant analysis). These profiles were classified as adipose, stromal and tumor tissues with 92.3% accuracy with a leave-one patient-out cross-validation. Tissue recognition using this ex vivo-built REIMS classification model was subsequently tested in vivo on electrosurgical vapors. Stromal and adipose tissues ...Continue Reading

References

Feb 10, 2010·Journal of Proteome Research·Stacey R OppenheimerRichard M Caprioli
Jul 19, 2013·Science Translational Medicine·Júlia BalogZoltán Takáts
Sep 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·David CalligarisNathalie Y R Agar
Jan 20, 2016·Oncotarget·Alastair J Ironside, J Louise Jones
Jan 7, 2017·Annals of Surgical Oncology·Richard J GraySarah Blair
Nov 22, 2017·PloS One·Josch K PaulingChrister S Ejsing
Jun 14, 2019·Analytical Chemistry·Emrys A JonesZoltan Takats

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Citations

May 10, 2021·Trends in Molecular Medicine·Nina OgrincIsabelle Fournier

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Methods Mentioned

BETA
PCA
biopsies

Software Mentioned

HD Imaging
ALEX
REIMS
AMX
QuPath

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