Strong c-Jun/AP-1 immunoreactivity is restricted to apoptotic cells following intracerebral ibotenic acid injection in developing rats

Neuroscience Research
I FerrerA M Planas

Abstract

Strong c-Jun immunoreactivity, as revealed with the antibody c-Jun/activator protein 1 (AP-1) which is raised against the amino acids 91-105 mapping with the amino terminal domain of mouse c-Jun p39, is observed in apoptotic cells, but not in necrotic cells, following intracerebral injection of ibotenic acid in the developing rat brain processed for immunohistochemistry. Immunostaining occurs in the cytoplasm and dendrites, thus suggesting impaired nuclear translocation of c-Jun in apoptotic cells. Western blotting of total brain homogenates, using the same antibody, shows a band at p39 which is more marked in treated animals than in age-matched controls. In addition, increased c-Jun N-terminal kinase 1 (JNK-1) expression, as revealed on Western blots, is found in rats treated with ibotenic acid when compared with controls. In contrast, apoptotic cells are not stained with antibodies to Jun B and Jun D. These results give further support to previous studies showing strong c-Jun expression in apoptotic cells at determinate stages of development, and emphasize that intracellular distribution of c-Jun, possible post-translational modifications of c-Jun due to phosphorylation at specific transactivation sites, and lack of associate...Continue Reading

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Citations

Feb 23, 2000·Neuroscience·I FerrerA M Planas
Nov 14, 1998·Brain Research·R A SheldonD M Ferriero
Aug 3, 2001·Seminars in Neonatology : SN·Z S Vexler, D M Ferriero
Jun 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joan RiberaJosep E Esquerda
Apr 19, 2002·Molecular Pharmacology·Subramaniam JayanthiJean Lud Cadet

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