Strong excitonic interactions in the oxygen-reducing site of bd-type oxidase: the Fe-to-Fe distance between hemes d and b595 is 10 A.

Biochemistry
Alexander M ArutyunyanA A Konstantinov

Abstract

Absorption and circular dichroism (CD) spectra of cytochrome bd from Escherichia coli have been compared for the wild type enzyme and an inactive mutant in which a highly conserved E445 in subunit I has been replaced by alanine [Zhang, J., Hellwig, P., Osborne, J. P., Huang, H. W., Moenne-Loccoz, P., Konstantinov, A. A., and Gennis, R. B. (2001) Biochemistry 40, 8548-8556]. The absorption bands of ferrous heme b595 are absent from the spectrum of the dithionite-reduced E445A form of cytochrome bd. The difference between the spectra of the dithionite-reduced WT and E445A enzymes indicates that in the mutant, heme b595 is present but is not reducible by dithionite. Cytochrome bd reveals intense CD signals dominated by heme d, with almost no contribution from heme b595 or heme b558. The CD spectrum of the reduced wild type enzyme in the Soret band indicates strong excitonic interactions between ferrous heme d and ferrous heme b595, and these interactions are not observed in dithionite-reduced E445A mutant, in which heme b595 remains in the ferric state. Modeling the excitonic interactions in both absorption and CD spectra has been carried out, yielding an estimate of the Fe-to-Fe distance between heme d and heme b595 of about 10 A...Continue Reading

References

Aug 2, 1993·FEBS Letters·I KrasnoselskayaA A Konstantinov
Apr 19, 1996·The Journal of Biological Chemistry·H HoriY Anraku

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Citations

Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Vitaliy B BorisovMichael I Verkhovsky
Jul 14, 2010·Biochemistry. Biokhimii︠a︡·V B BorisovA A Konstantinov
Feb 3, 2011·Metallomics : Integrated Biometal Science·Artem V DyubaAlexander A Konstantinov
Dec 3, 2014·Biochimica Et Biophysica Acta·Vitaliy B BorisovAlessandro Giuffrè
Nov 6, 2012·Journal of Inorganic Biochemistry·Vitaliy B Borisov, Michael I Verkhovsky
Mar 1, 2011·Biochimica Et Biophysica Acta·Vitaliy B BorisovAlessandro Giuffrè
Jul 16, 2011·Biochimica Et Biophysica Acta·Vitaliy B BorisovMichael I Verkhovsky
Jun 26, 2012·Biochimica Et Biophysica Acta·Alexander M ArutyunyanVitaliy B Borisov
Feb 4, 2014·Biochimica Et Biophysica Acta·Alessandro GiuffrèElena Forte
Jun 10, 2016·Scientific Reports·Sinan Al-AttarSimon de Vries
Nov 15, 2019·Nature Communications·Alexander TheßelingThorsten Friedrich
Mar 12, 2021·Biochemistry. Biokhimii︠a︡·Vitaliy B Borisov, Elena Forte
Aug 1, 2009·EcoSal Plus·Vitaliy B Borisov, Michael I Verkhovsky
Jan 7, 2016·EcoSal Plus·Vitaliy B Borisov, Michael I Verkhovsky

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