Strong Inhibitory Effects of Antisense Probes on Gene Expression through Ultrafast RNA Photocrosslinking

Chemistry, an Asian Journal
Kenzo FujimotoShigetaka Nakamura

Abstract

We have reported the photochemical regulation of the intracellular antisense effect of antisense probes containing a photo-responsive artificial nucleic acid, 3-cyanovinylcarbazole nucleoside (CNV K). Here we focus on the importance of the photocrosslinking rate on the inhibitory effect on gene expression using photocrosslinkable antisense probes (pcASOs). The inhibitory effect of pcASOs on GFP gene expression was dependent on the photocrosslinking rate of 3-cyanovinylcarbazole with d-threoninol (CNV D), CNV K, or psoralen. The ultrafast RNA photocrosslinking induced the formation of a thermally irreversible covalent bond between pcASOs and the target RNA. These ASOs strongly inhibited gene expression only when the photocrosslinking rate was faster than the random walk of branch migration. In addition, pcASOs containing CNV D or CNV K targeted the RNAs with secondary structures. These results indicate the regulatory effect of photocrosslinker and photoirradiation energy using pcASOs on the gene expression level.

References

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Jun 28, 2008·Organic Letters·Yoshinaga Yoshimura, Kenzo Fujimoto
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Aug 11, 2017·Chembiochem : a European Journal of Chemical Biology·Shigetaka NakamuraKenzo Fujimoto
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Mar 14, 2018·Scientific Reports·Rintaro Iwata HaraTakeshi Wada

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Citations

Jul 1, 2020·Chembiochem : a European Journal of Chemical Biology·Yasuha Watanabe, Kenzo Fujimoto
Jun 11, 2020·Chembiochem : a European Journal of Chemical Biology·Shigetaka NakamuraKenzo Fujimoto
Jan 25, 2021·Bioorganic & Medicinal Chemistry Letters·Kenzo FujimotoShigetaka Nakamura
Jul 12, 2021·Bioorganic & Medicinal Chemistry Letters·Ahmed Mostafa AbdelhadyFumi Nagatsugi
Oct 14, 2021·Chembiochem : a European Journal of Chemical Biology·Kenzo FujimotoShigetaka Nakamura
Jan 4, 2022·The Journal of Organic Chemistry·Ahmed Mostafa AbdelhadyFumi Nagatsugi

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