PMID: 9630694Jun 19, 1998Paper

Strong synergistic anti-peroxidative effects of HDL3 and ascorbic acid against copper-catalyzed LDL peroxidation

Biochimica Et Biophysica Acta
M YoshikawaY Kamiya

Abstract

The aim of this study was to investigate the effects of high density lipoprotein 3 (HDL3) and ascorbic acid (AsA) in combination on copper-catalyzed low density lipoprotein (LDL) peroxidation. LDL and HDL3 were isolated from sera of healthy volunteers. LDL protein, 200 microg/ml, was incubated in phosphate-buffered saline (PBS) containing 2.5 microM CuSO4 in the absence or presence of AsA, with HDL3 protein alone, or with coincubation of HDL3, 200 microg/ml, and AsA, 20 microg/ml, at 37 degrees C for up to 24 h. As a control, the same amount of control LDL protein was added to PBS. The protective effects of the HDL3 and AsA were examined by both electrophoresis and determination of the lipid hydroperoxide (LPO) level in each sample. The concentration of AsA was also measured in samples containing AsA. The coincubation of HDL3 and AsA exerts more powerful anti-peroxidative effects against copper-catalyzed LDL peroxidation, than either of these agents alone. In addition, AsA was retained in the media by the addition of HDL3. The findings suggest that there are strong synergistic anti-peroxidative effects of HDL3 and AsA and these two may act in concert in vivo to inhibit LDL peroxidation and thus exert an anti-atherosclerotic eff...Continue Reading

References

May 1, 1992·Atherosclerosis·D J Lamb, D S Leake
Jul 29, 1991·FEBS Letters·M I MacknessP N Durrington
Mar 1, 1991·Proceedings of the Society for Experimental Biology and Medicine·G C HunterC D Eskelson
Oct 1, 1990·Biochemical Pharmacology·R Chander, N K Kapoor
May 22, 1990·Biochimica Et Biophysica Acta·S ParthasarathyL G Fong
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B FreiB N Ames
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B FreiB N Ames
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M T QuinnD Steinberg
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M T QuinnD Steinberg
Jul 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·D W MorelG M Chisolm
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·U P SteinbrecherD Steinberg
Mar 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T HenriksenD Steinberg
Oct 1, 1980·The American Journal of Clinical Nutrition·J Ramirez, N C Flowers
Jun 1, 1995·Atherosclerosis·M I Mackness, P N Durrington
May 2, 1994·FEBS Letters·S P Gieseg, H Esterbauer
Apr 1, 1993·Atherosclerosis·A N KlimovN P Teryukova
Dec 1, 1995·Journal of the American College of Nutrition·M L SlatteryB J Caan
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

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Citations

Nov 28, 2009·Journal of Atherosclerosis and Thrombosis·Nagahiko SakumaSyuichi Kitada
Oct 2, 2003·The Journal of Nutrition·Robert J HillstromSean M Lynch

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