Strontium attenuates rhBMP-2-induced osteogenic differentiation via formation of Sr-rhBMP-2 complex and suppression of Smad-dependent signaling pathway

Acta Biomaterialia
Wenjing ZhangChangsheng Liu

Abstract

Strontium (Sr(2+)) has pronounced effects on stimulating bone formation and inhibiting bone resorption in bone regeneration. In this current study, the effect and the underlying mechanism involved of Sr(2+) on the biological activity of bone morphogenetic protein-2 (BMP-2) were studied in detail with pluripotent skeletal muscle myogenic progenitor C2C12 model cell line. The results indicated that Sr(2+) could bind recombinant human BMP-2 (rhBMP-2) rapidly, even in the presence of Ca(2+) and Mg(2+), and inhibited rhBMP-2-induced osteogenic differentiation in vitro and osteogenetic efficiency in vivo. Further studies demonstrated that Sr(2+) treatment undermined the binding capacity of rhBMP-2 with its receptor BMPRIA and thus attenuated Smad 1/5/8 phosphorylation without affecting their dephosphorylation in C2C12 cells. Furthermore, circular dichroism spectroscopy, fluorescence spectroscopy and X-ray photoelectron spectroscopy all revealed that the inhibitory effect of Sr(2+) on the rhBMP-2 osteogenic activity was associated with the formation of Sr-rhBMP-2 complex and ensuing enhancement of β-sheet structure. Our work suggests the activity of rhBMP-2 to induce osteogenic differentiation was decreased by directly interaction wit...Continue Reading

References

May 6, 2003·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Edward M Brown
Jul 9, 2003·Kidney International·Steven C VerberckmoesPatrick C D'Haese
Oct 31, 2003·Nature·Quyen Q HoangDaniel S C Yang
Apr 6, 2004·Nature Structural & Molecular Biology·Sascha KellerThomas D Mueller
Jul 4, 2006·Protein Science : a Publication of the Protein Society·Paola LlinasMarie Hélène Le Du
Mar 4, 2009·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Songlin PengWilliam W Lu
Jul 6, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ludmila LucaOlivier Jordan
Sep 24, 2013·Biochemical and Biophysical Research Communications·Ziyu LiChangsheng Liu
Jan 15, 2014·Acta Crystallographica. Section D, Biological Crystallography·Petri Kursula
Nov 21, 2014·Nature Communications·Chancievan ThangaratnarajahEdmund R S Kunji

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Citations

May 13, 2017·Journal of Biomedical Materials Research. Part a·M N BiaoB C Yang
Mar 31, 2017·Nanoscale·Yulin Li, Changsheng Liu
Nov 18, 2017·Stem Cell Research & Therapy·Rubén Aquino-MartínezFrancesc Ventura Pujol
Sep 13, 2018·Journal of Periodontal Research·Carolina Bizelli-SilveiraDavid C E Kraft
Dec 21, 2019·Dose-response : a Publication of International Hormesis Society·Yanxin LiuJinqiao Wang
Jan 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zheni GongZhihong Zhu
Jun 6, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·I GoñiM Gurruchaga
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Delu ZhaoMiao Yin
Aug 25, 2021·Journal of Materials Science. Materials in Medicine·Chunli ZhangZhonghai Li

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