Structural alterations and conformational dynamics in Holliday junctions induced by binding of a site-specific recombinase

Molecular Cell
J LeeM Jayaram

Abstract

Binding of a cleavage-incompetent mutant of the Flp recombinase induces a roughly square-planar geometry in synthetic immobile Holliday junctions. The branch points, which are rigidly fixed in these junctions in their free forms, tend to be more flexible in their protein-bound forms. Our results (1) suggest a plausible mechanism for the switching of the recombination complex from the Holliday-forming mode to the Holliday-resolving mode, (2) provide a rationale for previous observations that Flp resolves preformed immobile Holliday structures in the parental or in the recombinant mode in a relatively unbiased manner, and (3) accommodate two modes of DNA cleavage by Flp (transhorizontal or transdiagonal) in Holliday substrates.

References

Nov 20, 1990·Journal of Molecular Biology·C J Schwartz, P D Sadowski
May 11, 1990·Nucleic Acids Research·E von KitzingS Diekmann
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J P Cooper, P J Hagerman
Dec 20, 1987·Journal of Molecular Biology·J P Cooper, P J Hagerman
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M JayaramR M Harshey
Jan 1, 1988·Annual Review of Genetics·N L Craig
Oct 7, 1988·Cell·D R DuckettD M Lilley
Aug 9, 1988·Biochemistry·J H ChenN C Seeman
May 12, 1995·The Journal of Biological Chemistry·X D ZhuP D Sadowski
Aug 25, 1995·Journal of Molecular Biology·K H Luetke, P D Sadowski
Sep 15, 1995·Journal of Molecular Biology·R J Bennett, S C West
Jan 30, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D R DuckettD M Lilley
Oct 21, 1994·Journal of Molecular Biology·J E Dixon, P D Sadowski
Dec 5, 1993·Journal of Molecular Biology·J E Dixon, P D Sadowski
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P D Sadowski
Nov 1, 1995·Molecular Microbiology·J E DixonP D Sadowski
Nov 8, 1996·Journal of Molecular Biology·C Altona
Jun 1, 1997·The EMBO Journal·L K ArciszewskaD J Sherratt
Jun 27, 1997·Journal of Molecular Biology·M F WhiteD M Lilley

❮ Previous
Next ❯

Citations

Dec 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey P Mumm
Jun 17, 1999·Journal of Molecular Biology·N J CrisonaN R Cozzarelli
Jun 17, 1999·Journal of Molecular Biology·J BathS D Colloms
Feb 5, 1999·Journal of Molecular Biology·K E Huffman, S D Levene
Feb 27, 1999·Current Opinion in Structural Biology·D N Gopaul, G D Duyne

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.