PMID: 2118505Sep 1, 1990Paper

Structural alterations in the Bacillus subtilis Spo0A regulatory protein which suppress mutations at several spo0 loci

Journal of Bacteriology
G B SpiegelmanJ A Hoch

Abstract

Secondary site mutations that restore sporulation to sporulation-defective spo0F or spo0B deletion mutants were found to reside in the spo0A gene. Sequence analysis of 23 such sof mutants showed that the sof mutations fell into six classes of missense codon changes, primarily in the conserved amino-terminal domain of the response regulator Spo0A protein. Changes were observed in codons 12, 14, 60, 92, and 121. The residues affected were predominantly located in the potential turn regions at one end of the amino-terminal conserved domain on the same topological face as the active site aspartate residues. The ability of sof mutations to suppress deficiencies in the transmitter kinases, KinA and KinB, of two-component regulatory systems was tested. All of the sof mutations suppressed the sporulation deficiency of kinA mutants but only two classes among five tested suppressed kinB mutations. sof mutants segregated Spo- colonies at high frequency. Five of these Spo- mutants were found to result from mutations in the spo0A locus that reversed the effect of the sof mutatation. One of these was sequenced and found to have the original sof mutation and a new mutation, sos, at codon 105. The accumulation of sos mutations in sof strains s...Continue Reading

References

Dec 1, 1976·Bacteriological Reviews·P J Piggot, J G Coote
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jan 1, 1976·Advances in Genetics·J A Hoch
Dec 1, 1989·Microbiological Reviews·J B StockA M Stock
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A J Ninfa, B Magasanik
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K A TrachJ A Hoch
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B T NixonF M Ausubel
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F A FerrariJ A Hoch
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J F HessM I Simon
Sep 1, 1973·Journal of Bacteriology·S P BrehmJ A Hoch
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·P SchaefferJ P Aubert
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen
Jan 1, 1984·Molecular & General Genetics : MGG·R A SharrockT Leighton

❮ Previous
Next ❯

Citations

Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·K Stephenson, J A Hoch
Aug 26, 2017·The EMBO Journal·Jonathan R RussellRichard Losick
May 10, 2000·The Journal of Biological Chemistry·M A Cervin, G B Spiegelman
Jun 18, 2002·Molecular Microbiology·Sophie J Stephenson, Marta Perego
Jul 17, 2004·Molecular Microbiology·K MuchováI Barák
Jan 21, 2004·Molecular Microbiology·Jenny G SmithRobert B Bourret
Jan 6, 2011·Molecular Microbiology·Hanjing YangJeffrey H Miller
Oct 24, 2001·Journal of Bacteriology·J D QuiselA D Grossman
Jan 30, 2007·Journal of Bacteriology·Cristina BongiorniMarta Perego
Jul 1, 1992·Journal of Bacteriology·J P Mueller, A L Sonenshein
Sep 1, 1991·Research in Microbiology·B D GreenP Youngman
Apr 1, 1991·Journal of Bacteriology·M Perego, J A Hoch
Apr 1, 1993·Current Opinion in Genetics & Development·M A Strauch, J A Hoch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.