Structural analysis of a peptide--HLA class II complex: identification of critical interactions for its formation and recognition by T cell receptor

International Immunology
J B RothbardJ R Lamb

Abstract

An assay for the binding of peptides to major histocompatibility complex (MHC) class II proteins on the surface of cells has been used to determine the relative importance of the amino acids composing an influenza haemagglutinin T cell determinant in binding. The important contact residues were identified by the effect substitution of each residue with biotinylated lysine had on the ability of the peptide to bind. The spacing of the critical residues within the peptide sequence was consistent with the central core, of approximately eight amino acids, adopting a helical conformation. The terminal residues were less constrained and might not be part of a regular conformation. Increasing the helical propensity of the determinant, by simply acetylating and amidating the peptide, resulted in an analogue that was able to stimulate a specific T cell clone at significantly lower concentrations than the natural sequence. A potential location for the peptide in the binding site was postulated based on the presence of complementary amino acids in the class II molecule and supported by screening a large number of peptide analogues for their ability either to bind the restriction element or to stimulate T cell proliferation.

Citations

Nov 6, 1990·Journal of Immunological Methods·R Busch, J B Rothbard
Apr 1, 1993·Molecular Immunology·E F RosloniecJ H Freed
Jan 1, 1994·Molecular Immunology·Y AltuviaH Margalit
Apr 1, 2000·Biochimica Et Biophysica Acta·A ChersiL Rosanò
Jun 18, 2002·Immunology and Cell Biology·Anne S De GrootWilliam Martin
Jan 1, 1992·The Journal of Experimental Medicine·G RubertiA Livingstone
Oct 1, 1992·The Journal of Experimental Medicine·J HammerF Sinigaglia
Feb 1, 1995·The Journal of Experimental Medicine·F Sinigaglia, J Hammer
Mar 1, 1994·Annals of the Rheumatic Diseases·M A SkinnerP L Tan
Jun 1, 1997·Transplant Immunology·M Kumagai-BraeschJ T Kurnick
Apr 23, 2010·Immunology·Morten NielsenClaus Lundegaard
Dec 1, 1990·Molecular and Cellular Probes·A D Rees
Apr 25, 2008·Current Protocols in Immunology·J B Rothbard, R Busch
Jan 18, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason M GodAzizul Haque
Jan 1, 1990·International Reviews of Immunology·J B RothbardC M Hill
Feb 24, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F FerreiraO Scheiner
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C L VanderlugtS D Miller
Feb 26, 1999·International Reviews of Immunology·Y NishimuraS Matsushita
Aug 1, 1992·European Journal of Immunology·J K HicklingR Bülow
Apr 26, 2021·HLA : Immune Response Genetics·David J LangtonCarlos Echevarria

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