Structural analysis of alterations in zebrafish muscle differentiation induced by simvastatin and their recovery with cholesterol

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Laise M CamposManoel Luís Costa

Abstract

In vitro studies show that cholesterol is essential to myogenesis. We have been using zebrafish to overcome the limitations of the in vitro approach and to study the sub-cellular structures and processes involved during myogenesis. We use simvastatin--a drug widely used to prevent high levels of cholesterol and cardiovascular disease--during zebrafish skeletal muscle formation. Simvastatin is an efficient inhibitor of cholesterol synthesis that has various myotoxic consequences. Here, we employed simvastatin concentrations that cause either mild or severe morphological disturbances to observe changes in the cytoskeleton (intermediate filaments and microfilaments), extracellular matrix and adhesion markers by confocal microscopy. With low-dose simvastatin treatment, laminin was almost normal, and alpha-actinin was reduced in the myofibrils. With high simvastatin doses, laminin and vinculin were reduced and appeared discontinuous along the septa, with almost no myofibrils, and small amounts of desmin accumulating close to the septa. We also analyzed sub-cellular alterations in the embryos by electron microscopy, and demonstrate changes in embryo and somite size, septa shape, and in myofibril structure. These effects could be reve...Continue Reading

References

Apr 13, 1999·Cell and Tissue Research·U KaufmannA Starzinski-Powitz
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kathleen A ClarkCarol C Gregorio
Jun 20, 2003·Nature Reviews. Drug Discovery·Jonathan A Tobert
Feb 13, 2004·Developmental Cell·Juanita L ThorpeSteven A Farber
Nov 24, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M L CostaC S Mermelstein
Jul 12, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C S MermelsteinM L Costa
Oct 18, 2005·Developmental Biology·Clarissa A HenrySharon L Amacher
Dec 3, 2005·Developmental Biology·Steven M PollardDerek L Stemple
Mar 16, 2007·Differentiation; Research in Biological Diversity·Cláudia S MermelsteinJosé Garcia Abreu
Feb 12, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christian SchröterAndrew C Oates
May 2, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peirang CaoStewart H Lecker
May 22, 2010·The American Journal of Pathology·Annette DraegerEduard B Babiychuk
Feb 27, 2014·Journal of Biomedical Semantics·Ceri E Van SlykeMelissa A Haendel

❮ Previous
Next ❯

Citations

Nov 20, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Matthew J AllenBrigitte von Rechenberg
Feb 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jessica HoppstädterAlexandra K Kiemer
Feb 13, 2020·Npj Regenerative Medicine·Celia Herrera-RinconMichael Levin
Feb 4, 2021·Npj Regenerative Medicine·Celia Herrera-RinconMichael Levin
Jun 3, 2021·International Journal of Molecular Sciences·Magda Dubińska-MagieraKrzysztof Jagla

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
transmission electron microscopy
glycosylation
geranylgeranylation

Software Mentioned

Adobe Photoshop
NIH Image
ImageJ

Related Concepts

Related Feeds

Biomarkers for Cardiovascular Risk Assessment

Sensitive and accurate biomarkers used in cardiovascular risk prediction can potentially be used to manage the risk of cardiovascular disease. Discover the latest research on Biomarkers for Cardiovascular Risk Assessment here. Discover the latest research on Biomarkers for Cardiovascular Risk Assessment here.

Related Papers

Deutsche medizinische Wochenschrift
U Burgmer, K Mohr
Annals of Internal Medicine
Katarina LeBlanc, Mary T Brophy
Revue de l'infirmière
Corinne Taéron
© 2021 Meta ULC. All rights reserved