Structural analysis of biofilms and pellets of Aspergillus niger by confocal laser scanning microscopy and cryo scanning electron microscopy

Bioresource Technology
Gretty K VillenaMarcel Gutiérrez-Correa

Abstract

Biomass organization of Aspergillus niger biofilms and pellets stained with fluorescein isothiocyanate were analyzed by means of confocal laser scanning microscopy and detectable differences between both types of growth were found. Three-dimensional surface plot analysis of biofilm structure revealed interstitial voids and vertical growth compared with pellets. Growth was lower in biofilm and according to fluorescence profile obtained, biomass density increased at the surface (0-20 microm). However, a decrease in fluorescence intensity was observed through optical sections of pellets even though growth was significantly higher than biofilms. Cryo scanning electron microscopy also showed structural differences. While biofilms showed a spatially ordered mycelium and well structured hyphal channels, pellets were characterized by an entangled and notoriously compacted mycelium. These findings revealed common structural characteristics between A. niger biofilms and those found in other microbial biofilms. Thus, biofilm microstructure may represent a key determinant of biofilm growth and physiology of filamentous fungi.

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Citations

Apr 1, 2010·Applied Microbiology and Biotechnology·Norma N GamarraMarcel Gutiérrez-Correa
Feb 22, 2012·Applied Biochemistry and Biotechnology·Marcel Gutiérrez-CorreaGretty K Villena
Mar 22, 2012·Applied Biochemistry and Biotechnology·Sayani MitraJoydeep Mukherjee
Aug 2, 2011·Microbial Cell Factories·Thomas WucherpfennigRainer Krull
May 27, 2015·Biofouling·Lúcia Chaves SimõesNelson Lima
Dec 20, 2011·Fungal Genetics and Biology : FG & B·Bert-Ewald PriegnitzAndré Fleissner
Jul 10, 2012·Journal of Biotechnology·Rainer KrullChristoph Wittmann
Nov 19, 2011·FEMS Microbiology Letters·Gordon RamageCraig Williams
Apr 3, 2008·PloS One·Anders FolkessonSøren Molin
Sep 24, 2016·World Journal of Microbiology & Biotechnology·Eva KvasničkováJan Masák
Oct 21, 2016·BMC Microbiology·Alejandra Itzel González-RamírezMaría Angeles Martínez-Rivera
Feb 24, 2018·Applied Microbiology and Biotechnology·Lukas VeiterChristoph Herwig
Feb 16, 2019·Biotechnology and Bioengineering·Stefan SchmidederHeiko Briesen
May 28, 2019·The Journal of Microbiology·Itzel Margarita Córdova-AlcántaraAida Verónica Rodriguez-Tovar
Oct 21, 2011·Current Microbiology·Virginia Siqueira, Nelson Lima
Jul 21, 2017·Standards in Genomic Sciences·Sujay PaulMarcel Gutiérrez-Correa
Jan 27, 2018·Journal of Fungi·Caroline B Costa-OrlandiMaria José S Mendes-Giannini
Oct 31, 2019·AIMS Microbiology·Alexandra SoaresFilipe J Mergulhão
Feb 9, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·R A de Souza, N M Kamat

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