PMID: 9188845Feb 1, 1997Paper

Structural analysis of class I MHC molecules: the cytoplasmic domain is not required for cytoskeletal association, aggregation and internalization

Molecular Immunology
H GurP E Lipsky

Abstract

The role of the cytoplasmic domain in a variety of the functional activities of class I MHC molecules has not been documented. To address this question, Jurkat cells were transfected with genes for either native class I MHC molecules or constructs in which all but four cytoplasmic amino acids were deleted. Antibody-induced aggregation and internalization of class I MHC molecules were examined by flow cytometry, and cytoskeletal association was determined by analysing the detergent-resistant fraction of FITC-labeled mAb to class I molecules. The results indicate that the truncated class I MHC molecules are comparable to native class I MHC molecules in the ability to move in the plane of the membrane and aggregate, to associate with the cytoskeleton and to undergo mAb-induced internalization at 37 degrees C. Thus, the cytoplasmic domain of class I MHC molecules is not required for these functional activities.

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Citations

Jan 18, 2005·Immunogenetics·Paula P CardenasManuel A Patarroyo
Nov 5, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A E PedersenM H Claesson
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M StoryH L Ploegh
Mar 20, 2013·European Journal of Haematology·Alessandro AllegraCaterina Musolino
Feb 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bhavna VermaJon A Weidanz
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neeraj TiwariFrank Momburg
Dec 8, 2006·The Biochemical Journal·Bryce W CareyDora M Kovacs

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