Structural analysis of DegS, a stress sensor of the bacterial periplasm

FEBS Letters
Kornelius Zeth

Abstract

Regulated proteolysis is a key event in transmembrane signalling between intracellular compartments. In Escherichia coli the membrane-bound protease DegS has been identified as the periplasmic stress sensor for unfolded outer membrane proteins (OMPs). DegS inititates a proteolytic cascade resulting in the release of sigmaE the transcription factor of periplasmic genes. The crystal structure of DegS protease reported at 2.2 A resolution reveals a trimeric complex with the monomeric protease domain in an inhibited state followed by the inhibitory PDZ domain. Noteably, domain architecture and communication of DegS are remarkably to homologous proteins known to date. Here the domain interface is mechanically locked by three intradomain salt bridges. Co-crystallisation trials in the presence of a 10-residue activating peptide did not result in significant structural intradomain shifts nor distortions in the crystal packing. These observations imply a mode of activation indicative of peptide-induced structural shifts imposed to the protease domain rather than disturbing the PDZ-protease interface.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S Brown, J L Goldstein
Jul 6, 2000·Nature Structural Biology·A W KarzaiR T Sauer
Nov 28, 2000·Acta Crystallographica. Section D, Biological Crystallography·A Vagin, A Teplyakov
Nov 1, 2002·Molecular Cell·Tim ClausenMichael Ehrmann
Feb 11, 2003·Structure·Raimond B G RavelliSean McSweeney
Nov 25, 2003·The EMBO Journal·Kazue KaneharaYoshinori Akiyama
Dec 31, 2003·Methods in Enzymology·Richard J MorrisVictor S Lamzin
Mar 3, 2004·Acta Crystallographica. Section D, Biological Crystallography·Laurent C StoroniRandy J Read
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

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Citations

Aug 2, 2012·Cell Stress & Chaperones·Dorota Zurawa-JanickaBarbara Lipinska
Jan 5, 2008·Cell Death and Differentiation·L Vande WalleP Vandenabeele
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jiansen JiangSen-Fang Sui
Aug 27, 2010·The Journal of Biological Chemistry·Jungsan SohnRobert T Sauer
Sep 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Petra Wollmann, Kornelius Zeth
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Ross E DalbeyJan Maarten van Dijl
Dec 4, 2012·Nature Chemical Biology·Randall V Mauldin, Robert T Sauer
Jul 19, 2012·Cellular and Molecular Life Sciences : CMLS·Guido Hansen, Rolf Hilgenfeld
Dec 26, 2015·Biochimica Et Biophysica Acta·Miroslaw JarzabBarbara Lipinska
May 18, 2010·Expert Opinion on Therapeutic Targets·Dorota Zurawa-JanickaBarbara Lipinska
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Anna K de RegtRobert T Sauer
Nov 6, 2008·Current Opinion in Microbiology·Sarah E Ades
Aug 19, 2007·Journal of Molecular Biology·Petra Wollmann, Kornelius Zeth
Jun 28, 2005·Molecular Microbiology·Jesper E Mogensen, Daniel E Otzen
Oct 27, 2007·Protein Science : a Publication of the Protein Society·Steven T RunyonSachdev S Sidhu
Mar 21, 2009·Molecular Microbiology·Brent O Cezairliyan, Robert T Sauer
Sep 19, 2006·Journal of Molecular Biology·Konstantin V KorotkovWim G J Hol
Nov 6, 2007·Cell·Jungsan SohnRobert T Sauer
Mar 13, 2019·Journal of Bacteriology·Elizabeth M HartThomas J Silhavy
Jun 28, 2019·Frontiers in Microbiology·Xie XieMingdong Huang
Jan 17, 2020·Microbiological Research·Noordiyanah Nadhirah RoslanSuriana Sabri
Jun 3, 2010·Archives of Biochemistry and Biophysics·Anna Sobiecka-SzkatulaJoanna Skorko-Glonek
Aug 1, 2009·EcoSal Plus·Dawn M Macritchie, Tracy L Raivio

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