PMID: 6161373Nov 1, 1980Paper

Structural analysis of factor VIII antigen in von Willebrand disease

Proceedings of the National Academy of Sciences of the United States of America
R L NachmanW T Brown

Abstract

The Factor VIII antigen molecules in the plasma of patients with classical type 1 and variant type 2A von Willebrand disease were compared to the Factor VIII antigen molecules in normal plasma. Factor VIII antigen was isolated from plasma by solid-phase immunoprecipitation and analyzed by NaDodSO4/polyacrylamide gel electrophoresis; the stained Factor VIII antigen bands were removed, radioiodinated, and subjected to tryptic digestion. Computerized analysis of autoradiographs revealed that the two-dimensional peptide maps of the different Factor VIII antigens were remarkably similar. The results suggest that the Factor VIII antigen molecules in these two forms of von Willebrand disease are probably identical to the Factor VIII antigen molecules present in normal plasma. It is thus likely that the differences observed in plasma Factor VIII antigen in classical and variant von Willebrand disease are not due to qualitatively abnormal molecules but rather represent quantitative shifts in the metabolism of normal Factor VIII antigen molecules.

References

May 5, 1977·The New England Journal of Medicine·H R GralnickB S Coller
Sep 1, 1977·British Journal of Haematology·I Shoa'iD Meyer
Jul 1, 1978·Thrombosis Research·J A van Mourik, P A Bolhuis
Dec 13, 1979·The New England Journal of Medicine·T S ZimmermanD Meyer
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T S ZimmermanT S Edgington
Jul 1, 1976·British Journal of Haematology·M J WeinsteinE W Davie
Aug 8, 1975·Science·C A Converse, D S Papermaster
Sep 1, 1975·The Journal of Clinical Investigation·E A Jaffe, R L Nachman
Jan 1, 1974·Thrombosis Research·J A van MourikI A Mochtar
Mar 1, 1974·British Journal of Haematology·P B KernoffC R Rizza
Jul 18, 1974·The New England Journal of Medicine·I R PeakeJ C Giddings
Feb 27, 1980·Biochemical and Biophysical Research Communications·R L NachmanB Ferris
Feb 1, 1965·Analytical Biochemistry·C B LAURELL

❮ Previous
Next ❯

Citations

Jan 1, 1986·Veterinary Immunology and Immunopathology·R E Benson, W J Dodds
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A Fulcher, T S Zimmerman
Nov 1, 1982·The Journal of Clinical Investigation·Z M RuggeriT S Zimmerman
Jul 26, 2011·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Fabien BornertAhmed Feki
Jan 24, 2013·Clinical Chemistry·Veronica H FloodUNKNOWN Zimmerman Program Investigators
May 23, 2015·Haemophilia : the Official Journal of the World Federation of Hemophilia·C Millar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.