PMID: 11906606Mar 22, 2002Paper

Structural analysis of fertilin(beta) cyclic peptide mimics that are ligands for alpha6beta1 integrin

The Journal of Peptide Research : Official Journal of the American Peptide Society
H Li, N S Sampson

Abstract

The NMR structural analysis of two fertilin(beta) mimics cyclo(EC2DC1)YNH2, 1, and cyclo(D2EC2D1C1)YNH2, 2 is described. Both of these mimics are moderate inhibitors of sperm-egg binding with IC50 values of 500 microm in a mouse in vitro fertilization assay. For peptide 1, the optimized conformations that best match the NMR data have a pseudo-type II' beta-turn with the linker and Glu at the i+1 and i+2 positions, respectively. The EC2D1C1 sequence is in a nonclassical (type IV) beta-turn. For peptide 2, the conformation that best matches the NMR data has two turns: a pseudo-type II' beta-turn in the D2EC2D1 sequence followed by a nonclassical beta-turn in the EC2D1C1 sequence. The Cbeta-Cbeta distance between E and D1 in peptide 1 is 9.1 A, in peptide 2, it is 7.7 A. Thus, one possibility for the high IC50 values of these cyclic peptides is that the acidic residues are not constrained to a sufficiently tight turn, and thus much entropy must still be lost upon binding to the alpha6beta1 integrin. This explains why the cyclic peptides are the same as linear peptides at inhibiting sperm-egg binding.

References

May 29, 1992·Journal of Medicinal Chemistry·P L BarkerM T Lipari
Jan 1, 1985·Advances in Protein Chemistry·G D RoseJ A Smith
Feb 1, 1995·Molecular Reproduction and Development·J P EvansG S Kopf
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Jun 13, 2000·The Journal of Cell Biology·B J MillerD G Myles
Apr 26, 2001·Developmental Biology·H NishimuraP Primakoff

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