Structural analysis of glucosylceramides (GlcCer) from species of the Pseudallescheria/Scedosporium complex

Fungal Biology
Renata O R CalixtoEliana Barreto-Bergter

Abstract

Glucosylceramides (GlcCer) are the main neutral glycosphingolipids expressed in fungal cells. In this work, glucosylceramides (GlcCer) were extracted from three strains of Scedosporium (Pseudallescheria) boydii, one strain of Pseudallescheria ellipsoidea and one strain of Pseudallescheria angusta and purified by several chromatographic steps. Using high-performance thin layer chromatography (HPTLC), we found a similarity between GlcCer obtained from all of the analysed strains. A detailed structural analysis of the P. ellipsoidea GlcCer was performed via electrospray ionization mass spectrometry (ESI-MS) and confirmed in 1- and 2-D heteronuclear NMR experiments ((1)H-(13) C HSQC). GlcCer species produced by mycelial forms of these strains displayed the same structure previously demonstrated by our group for P. boydii, Cryptococcus neoformans, Pseudallescheria minustipora, Fusarium solani, and Colletotrichum gloesporioides. A monoclonal antibody (mAb) against GlcCer was used for immunofluorescence experiments. Our results revealed that GlcCer is present on the surface of these fungi, and no difference was observed in the GlcCer structure of the present set of strains in terms of geographic or clinical origin, suggesting a conser...Continue Reading

References

Jan 1, 1987·Chemistry and Physics of Lipids·T MatsubaraY Nozawa
May 2, 1998·Biochimica Et Biophysica Acta·R S DuarteE B Bergter
Sep 11, 2002·Archives of Biochemistry and Biophysics·Daniela M MacielEliana Barreto-Bergter
Jun 3, 2006·The Journal of Clinical Investigation·Philipp C RittershausMaurizio Del Poeta
Jul 25, 2008·Medical Mycology·Fèlix GilgadoJosep Guarro
Dec 14, 2011·Frontiers in Microbiology·Eliana Barreto-BergterLauro M de Souza
Feb 1, 2012·Antimicrobial Agents and Chemotherapy·Michaela LacknerJacques F Meis
Jul 30, 2014·Antimicrobial Agents and Chemotherapy·M LacknerG S de Hoog
Oct 30, 2014·Frontiers in Cellular and Infection Microbiology·Eliana Barreto-Bergter, Rodrigo T Figueiredo

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Citations

Jun 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Edson Rodrigues VieiraGalba Maria de Campos-Takaki
Sep 30, 2020·Mycopathologia·Rodrigo Rollin-PinheiroEliana Barreto-Bergter

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