Structural analysis of major species barriers between humans and palm civets for severe acute respiratory syndrome coronavirus infections.

Journal of Virology
Fang Li


It is believed that a novel coronavirus, severe acute respiratory syndrome coronavirus (SARS-CoV), was passed from palm civets to humans and caused the epidemic of SARS in 2002 to 2003. The major species barriers between humans and civets for SARS-CoV infections are the specific interactions between a defined receptor-binding domain (RBD) on a viral spike protein and its host receptor, angiotensin-converting enzyme 2 (ACE2). In this study a chimeric ACE2 bearing the critical N-terminal helix from civet and the remaining peptidase domain from human was constructed, and it was shown that this construct has the same receptor activity as civet ACE2. In addition, crystal structures of the chimeric ACE2 complexed with RBDs from various human and civet SARS-CoV strains were determined. These structures, combined with a previously determined structure of human ACE2 complexed with the RBD from a human SARS-CoV strain, have revealed a structural basis for understanding the major species barriers between humans and civets for SARS-CoV infections. They show that the major species barriers are determined by interactions between four ACE2 residues (residues 31, 35, 38, and 353) and two RBD residues (residues 479 and 487), that early civet SA...Continue Reading


Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Apr 16, 2003·The New England Journal of Medicine·Nelson LeeJoseph J Y Sung
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
May 6, 2003·Science·Marco A MarraRachel L Roper
Dec 4, 2003·Biochemical and Biophysical Research Communications·Xiaodong XiaoDimiter S Dimitrov
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Feb 3, 2004·The Journal of Biological Chemistry·Paul TowlerMichael W Pantoliano
Apr 23, 2004·The New England Journal of Medicine·Ignatius T S YuTommy Ho
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Nanshan Zhong
Oct 27, 2004·Emerging Infectious Diseases·Guodong LiangUNKNOWN SARS Diagnosis Working Group
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Huai-Dong SongGuo-Ping Zhao
Mar 26, 2005·The EMBO Journal·Wenhui LiMichael Farzan
Oct 14, 2006·Advances in Experimental Medicine and Biology·Fang LiStephen C Harrison
Apr 25, 2007·Virus Research·Zhengli Shi, Zhihong Hu
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯


Oct 13, 2010·Nature Reviews. Microbiology·Kim M PepinJames O Lloyd-Smith
Nov 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kailang WuFang Li
Feb 1, 2012·The Journal of Biological Chemistry·Kailang WuFang Li
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Colin R ParrishPeter Daszak
Sep 6, 2013·Annual Review of Genomics and Human Genetics·Cadhla Firth, W Ian Lipkin
Oct 2, 2013·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·R A PereraG Kayali
Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yang YangFang Li
Mar 24, 2012·Current Opinion in Virology·Denis Gerlier
Oct 6, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Chi Wai YipFrederick Chi-Ching Leung
Mar 19, 2013·Zoonoses and Public Health·R E KahnJ A Richt
Sep 26, 2014·The Journal of General Virology·Brenna McGruder, Julian L Leibowitz
Sep 1, 2016·Annual Review of Virology·Fang Li
Apr 1, 2020·Nature·Jian ShangFang Li
Apr 30, 2020·Journal of Biomolecular Structure & Dynamics·Hussain Mustatab WahediSumra Wajid Abbasi
May 18, 2020·The FEBS Journal·Alexander WlodawerMariusz Jaskolski
Aug 29, 2020·Nanoscale Research Letters·Sahel N AbduljauwadHabib-Ur-Rehman Ahmed
Aug 26, 2020·Pathogens and Disease·Jitendra Singh Rathore, Chaitali Ghosh
Sep 29, 2020·Scientific Reports·Greg N Brooke, Filippo Prischi
Jun 6, 2020·Human Genomics·Cathleen LutzWonyoung Kang
Aug 12, 2020·Cellular and Molecular Life Sciences : CMLS·Jie WuXiuhong Yang
Feb 18, 2020·Virus Evolution·Weng M NgThomas A Bowden
Jun 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yingjie WangJiali Gao
Dec 12, 2018·Nature Reviews. Microbiology·Jie CuiZheng-Li Shi
Apr 9, 2020·Frontiers in Microbiology·Ning WangLanying Du
Dec 8, 2020·Clinical Science·Fiona J WarnerChandana B Herath
Dec 22, 2020·PLoS Biology·Carina ConceicaoDalan Bailey
Jun 24, 2020·Annual Review of Pharmacology and Toxicology·Hariharan SivaramanJ Sivaraman
Dec 1, 2020·Computational and Structural Biotechnology Journal·Jaswinder SinghBaljit Singh

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.