Structural analysis of permethylated oligosaccharides using electrospray ionization quadrupole time-of-flight tandem mass spectrometry and deutero-reduction

Rapid Communications in Mass Spectrometry : RCM
Willy MorelleJean-Claude Michalski

Abstract

Deutero-reduced permethylated oligosaccharides were analyzed by electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) using a hybrid quadrupole orthogonal acceleration time-of-flight mass spectrometer, fitted with a nanoflow ESI source. Under these ionization conditions such derivatives preferentially form sodiated molecular species in addition to protonated molecular species. Under collision-induced dissociation, protonated and sodiated molecular species yield simple and predictable fragment mass spectra. A systematic study was conducted on a series of deutero-reduced permethylated glycans to allow rationalization of the fragmentation processes. MS/MS spectra were characterized by fragments resulting from the cleavage of glycosidic bonds. These fragments originating from both the reducing and the non-reducing ends of the glycan yield information on sequence and branching. Furthermore, the substituent 3-linked to a HexNAc unit was readily eliminated. Special attention was devoted to a systematic study of fucosylated glycans. The fucosylated deutero-reduced permethylated glycans were submitted to an acidic hydrolysis, releasing specifically the fucosyl residues. The nascent free hydroxyl groups ...Continue Reading

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Citations

Aug 28, 2007·Journal of the American Society for Mass Spectrometry·Catherine E CostelloJohn F Cipollo
Oct 16, 2007·Journal of the American Society for Mass Spectrometry·Michael PikulskiJennifer S Brodbelt
Jun 24, 2009·Journal of the American Society for Mass Spectrometry·Ming LeiMilos V Novotny
Jun 23, 2007·Nature Protocols·Willy Morelle, Jean-Claude Michalski
Oct 5, 2013·Glycobiology·Guillaume ChevreuxNicolas Bihoreau
Oct 27, 2007·Proteomics·Magali AndréFrançois Le Naour
Jun 21, 2006·Proteomics·Willy MorelleJean-Claude Michalski
Mar 28, 2007·Rapid Communications in Mass Spectrometry : RCM·Arugadoss DevakumarJames P Reilly
Mar 2, 2010·Journal of Proteomics·Kriangsak SongsriroteJane Thomas-Oates
Dec 1, 2007·Journal of Proteome Research·James A AtwoodRon Orlando

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