PMID: 9443975Mar 14, 1998Paper

Structural analysis of slipped-strand DNA (S-DNA) formed in (CTG)n. (CAG)n repeats from the myotonic dystrophy locus

Nucleic Acids Research
Christopher E PearsonR R Sinden

Abstract

The mechanism of disease-associated trinucleotide repeat length variation may involve slippage of the triplet-containing strand at the replication fork, generating a slipped-strand DNA structure. We recently reported formation in vitro of slipped-strand DNA (S-DNA) structures when DNAs containing triplet repeat blocks of myotonic dystrophy or fragile X diseases were melted and allowed to reanneal to form duplexes. Here additional evidence is presented that is consistent with the existence of S-DNA structures. We demonstrate that S-DNA structures can form between two complementary strands containing equal numbers of repeats. In addition, we show that both the propensity for S-DNA formation and the structural complexity of S-DNAs formed increase with increasing repeat length. S-DNA structures were also analyzed by electron microscopy, confirming that the two strands are slipped out of register with respect to each other and confirming the structural polymorphism expected within long tracts of trinucleotide repeats. For (CTG)50.(CAG)50 two distinct populations of slipped structures have been identified: those involving </=10 repeats per slippage, which appear as bent/kinked DNA molecules, and those involving >10 repeats, which hav...Continue Reading

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Citations

Apr 3, 2002·Journal of Biosciences·Richard R SindenLuda S Shlyakhtenko
Apr 1, 2004·Indian Journal of Pediatrics·Monisha Mukherjee, Balraj Mittal
Oct 16, 2002·Mutation Research·Vera I Hashem, Richard R Sinden
Jul 17, 1998·Current Opinion in Structural Biology·C E Pearson, R R Sinden
Aug 27, 2013·Chemical Reviews·Boris P BelotserkovskiiPhilip C Hanawalt
Aug 3, 2010·Nature Chemical Biology·Guoqi LiuMichael Leffak
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sharon F EdwardsRichard R Sinden
Oct 23, 2012·The Journal of Biological Chemistry·Gagan B PanigrahiChristopher E Pearson
Mar 8, 2011·Human Molecular Genetics·Stéphanie ToméChristopher E Pearson
Jan 9, 2013·Human Molecular Genetics·Laura W DillonYuh-Hwa Wang
Oct 18, 2002·Nucleic Acids Research·Christopher E PearsonKerrie Nichol
Dec 4, 2004·Nucleic Acids Research·Vera I HashemRichard R Sinden
Mar 16, 2005·Nucleic Acids Research·Lai Man Chi, Sik Lok Lam
Jul 26, 2005·Nucleic Acids Research·Samir AmraneJean-Louis Mergny
Jan 13, 2006·Nucleic Acids Research·Gema López-TorrejónSilvia Ayora
Nov 6, 2010·Nucleic Acids Research·Kaalak ReddyChristopher E Pearson
Mar 26, 2013·Nucleic Acids Research·Long MaDavid T F Dryden
Feb 23, 2012·Molecular and Cellular Biology·Guoqi LiuMichael Leffak
Oct 1, 2003·Molecular and Cellular Biology·Eitan ZlotorynskiBatsheva Kerem
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·J VölkerK J Breslauer
Aug 26, 2014·Nucleic Acids Research·Kaalak ReddyChristopher E Pearson
Jan 23, 2015·Critical Reviews in Biochemistry and Molecular Biology·Karen UsdinCatherine H Freudenreich
Jan 18, 2016·DNA Repair·Monika H M Schmidt, Christopher E Pearson
Jul 2, 2009·Journal of the American Chemical Society·Jens VölkerKenneth J Breslauer
Oct 13, 2015·Acta Crystallographica. Section D, Biological Crystallography·Arunachalam ThirugnanasambandamNamasivayam Gautham

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