PMID: 7545758Sep 15, 1995Paper

Structural analysis of the Bacillus subtilis delta factor: a protein polyanion which displaces RNA from RNA polymerase

Journal of Molecular Biology
F J López de SaroJ D Helmann

Abstract

RNA polymerase from Bacillus subtilis is a complex mixture comprising a common core (beta beta' alpha 2), the 20.4 kDa delta (delta) protein, and of one of several sigma (sigma) specificity factors. The delta protein, together with several truncated variants, has been overproduced and purified from Escherichia coli. It is highly acidic (pI = 3.6) and contains two distinct regions, a 13 kDa amino-terminal domain with fairly uniform charge distribution and a glutamate and aspartate residue-rich carboxyl-terminal region. The purified amino-terminal domain (delta N) contains 32% alpha-helix and 16% beta-sheet, as judged by circular dichroism analysis. In contrast, an 8.5 kDa tryptic fragment containing the carboxyl-terminal region (delta C) is largely unstructured and highly charged (net charge of -47). RNA polymerase purified from a B. subtilis mutant with an insertion in the delta gene (rpoE::cat) contains a truncated delta protein, indicating that the amino-terminal domain is stable in vivo and contains a core-binding function. Addition of delta, but not sigma A or delta N, displaces RNA bound to RNA polymerase in a binary complex. The ability of delta to displace RNA efficiently requires the activities of both the amino-termina...Continue Reading

Citations

Aug 6, 2014·Journal of Bacteriology·Andrew N KellerPeter J Lewis
May 18, 2016·Genome Biology and Evolution·Nicholas W V ChurtonJeremy S Webb
Oct 27, 2017·Journal of Biomolecular NMR·Pavel SrbLukáš Žídek
Jul 23, 2013·Chembiochem : a European Journal of Chemical Biology·Veronika PapouškováLukáš Žídek
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·Yong Heon LeeJohn D Helmann
May 21, 1999·The Journal of Biological Chemistry·F J López de SaroJ D Helmann
Jun 24, 2003·Infection and Immunity·Amanda L JonesCraig E Rubens
Jan 22, 2011·Critical Reviews in Biochemistry and Molecular Biology·Sung-Hoon JunKatsuhiko S Murakami
Apr 17, 2015·FEMS Microbiology Reviews·Andy Weiss, Lindsey N Shaw
Jan 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Brett M BabinDavid A Tirrell
Feb 13, 2014·Nucleic Acids Research·Jana WiedermannováLibor Krásny
Feb 12, 2014·Journal of Biomolecular NMR·Pavel KadeřávekLukáš Žídek
Nov 8, 2015·The Journal of Biological Chemistry·Ranjit Kumar PrajapatiJayanta Mukhopadhyay
Jul 19, 2012·Journal of Biomolecular NMR·Anna Zawadzka-KazimierczukMartin Billeter
Sep 30, 2016·The Journal of Biological Chemistry·Ranjit Kumar PrajapatiJayanta Mukhopadhyay
Aug 18, 2021·Transcription·Michael MillerPeter J Lewis
Dec 17, 2008·Methods : a Companion to Methods in Enzymology·John D Helmann

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