Structural analysis of the chromosome segregation protein Spo0J from Thermus thermophilus

Molecular Microbiology
Thomas A LeonardJan Löwe

Abstract

Prokaryotic chromosomes and plasmids encode partitioning systems that are required for DNA segregation at cell division. The plasmid partitioning loci encode two proteins, ParA and ParB, and a cis-acting centromere-like site denoted parS. The chromosomally encoded homologues of ParA and ParB, Soj and Spo0J, play an active role in chromosome segregation during bacterial cell division and sporulation. Spo0J is a DNA-binding protein that binds to parS sites in vivo. We have solved the X-ray crystal structure of a C-terminally truncated Spo0J (amino acids 1-222) from Thermus thermophilus to 2.3 A resolution by multiwavelength anomalous dispersion. It is a DNA-binding protein with structural similarity to the helix-turn-helix (HTH) motif of the lambda repressor DNA-binding domain. The crystal structure is an antiparallel dimer with the recognition alpha-helices of the HTH motifs of each monomer separated by a distance of 34 A corresponding to the length of the helical repeat of B-DNA. Sedimentation velocity and equilibrium ultracentrifugation studies show that full-length Spo0J exists in a monomer-dimer equilibrium in solution and that Spo0J1-222 is exclusively monomeric. Sedimentation of the C-terminal domain of Spo0J shows it to b...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·C O Pabo, R T Sauer
Apr 1, 1984·Journal of Bacteriology·H L MobleyU N Streips
Apr 1, 1995·Protein Engineering·M SuzukiM Gerstein
Jan 5, 1993·Journal of Molecular Biology·G D Van DuyneJ Clardy
Jan 1, 1993·Protein Engineering·G J Barton
Jun 20, 1993·Journal of Molecular Biology·R A LaskowskiJ M Thornton
Nov 1, 1995·Trends in Biochemical Sciences·L Holm, C Sander
Feb 1, 1997·Current Opinion in Biotechnology·W F Stafford
Jan 23, 1999·Science·O RodionovM Yarmolinsky
Mar 31, 1999·Trends in Genetics : TIG·M E Sharpe, J Errington
May 13, 1999·Molecular Microbiology·G Jagura-BurdzyC M Thomas
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·R B Jensen, L Shapiro
Mar 14, 2000·The Journal of Biological Chemistry·J Y BouetB E Funnell
Jul 6, 2000·Trends in Microbiology·J Møller-JensenK Gerdes
Aug 10, 2000·Molecular Microbiology·K GerdesR Bugge Jensen
Aug 31, 2000·Cell·R Heald
Sep 19, 2000·Nature·D J SharpJ M Scholey
Oct 6, 2000·Annual Review of Microbiology·G S Gordon, A Wright
Nov 28, 2000·Annual Review of Genetics·S Hiraga
Dec 6, 2000·Genome Biology·N M LuscombeJ M Thornton
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y Yamaichi, H Niki
Jan 21, 2001·The Journal of Biological Chemistry·J A Surtees, B E Funnell
Feb 13, 2001·Molecular Cell·K P Lemon, A D Grossman
Jun 13, 2001·Molecular Microbiology·J Sawitzke, S Austin
Aug 21, 2001·Genes & Development·K P Lemon, A D Grossman

❮ Previous
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Citations

Jan 18, 2006·Nature Reviews. Microbiology·Finbarr Hayes, Daniela Barillà
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simon RinggaardKenn Gerdes
Apr 24, 2012·Journal of Bacteriology·Fanny M PassotFranck Pasta
May 3, 2011·Journal of Bacteriology·Magdalena KusiakGrazyna Jagura-Burdzy
Feb 19, 2013·Journal of Bacteriology·Katherine CellerGilles P van Wezel
May 4, 2005·Journal of Bacteriology·Dagmara JakimowiczKeith F Chater
Jun 8, 2006·Annual Review of Biochemistry·Katharine A Michie, Jan Löwe
Jul 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Konstantin Doubrovinski, Martin Howard
Jun 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Chase P BroederszNed S Wingreen
Dec 3, 2014·Current Opinion in Microbiology·Stephan Gruber
Apr 21, 2010·Plasmid·Virginia S LioyJuan C Alonso
Jul 28, 2005·Molecular Microbiology·Yanina R SevastsyanovichChristopher M Thomas
Aug 24, 2006·Molecular Microbiology·Heath MurrayJeff Errington
Jun 1, 2012·Molecular Microbiology·Dagmara Jakimowicz, Gilles P van Wezel
Oct 20, 2012·Cellular Microbiology·Jerod L Ptacin, Lucy Shapiro
Feb 22, 2012·Molecular Microbiology·Catriona DonovanMarc Bramkamp
Dec 18, 2013·MicrobiologyOpen·Amanda P UcciHenrique Ferreira
Feb 24, 2006·Biochemical and Biophysical Research Communications·Mon-Juan LeeHaimei Huang
Apr 6, 2006·Trends in Biochemical Sciences·Finbarr Hayes, Daniela Barillà
May 14, 2005·Journal of Molecular Biology·Lewis E H BingleChristopher M Thomas
Nov 16, 2005·Annual Review of Genetics·Gitte Ebersbach, Kenn Gerdes
May 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Bo-Wei ChenYuh-Ju Sun
Aug 16, 2011·Journal of Molecular Biology·Barnali N Chaudhuri, Rebecca Dean
Jan 13, 2015·Nucleic Acids Research·James A TaylorMark S Dillingham
Jul 28, 2016·Trends in Microbiology·Daniela Barillà
Sep 14, 2016·Frontiers in Molecular Biosciences·Barbara E Funnell
Sep 27, 2016·Frontiers in Molecular Biosciences·María A Oliva
Nov 25, 2005·Nature·Maria A Schumacher, Barbara E Funnell
Apr 14, 2017·Nucleic Acids Research·Dan SongJoseph J Loparo

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