PMID: 8586629May 1, 1995Paper

Structural analysis of the L-methionine gamma-lyase gene from Pseudomonas putida

Journal of Biochemistry
H InoueH Tanaka

Abstract

The gene encoding L-methionine gamma-lyase from Pseudomonas putida was cloned and the primary structure of the enzyme was deduced from its nucleotide sequence. The L-methionine gamma-lyase gene was expressed in Escherichia coli. The amino acid sequences of BrCN-digested peptides agreed with the corresponding parts of the L-methionine gamma-lyase sequence determined from the gene structure. The polypeptide is composed of 398 amino acid residues with a calculated molecular weight of 42,626, corresponding to the subunit of the homotetrameric enzyme. The deduced amino acid sequence of L-methionine gamma-lyase only showed extensive homology with other well known alpha,gamma-elimination and/or gamma-replacement pyridoxal 5'-phosphate-dependent enzymes, such as cystathionine gamma-lyase, cystathionine gamma-synthase, and O-acetylhomoserine O-acetylserine sulfhydrylase, that participate in the biosynthesis of sulfur amino acids. However, the deduced essential cysteine residue of L-methionine gamma-lyase was not conserved in these enzymes. We confirmed the presence of a part of an open reading frame in the 3'-flanking region of the L-methionine gamma-lyase gene, which showed high homology with the N-terminal region of pyruvate dehydroge...Continue Reading

Citations

Apr 18, 2006·Biochemistry. Biokhimii︠a︡·I V ManukhovG B Zavilgelsky
Dec 6, 2006·Journal of Animal Science·P D LeM W A Verstegen
Jun 1, 2005·Nutrition Research Reviews·Phung D LeMartin W A Verstegen
Sep 1, 2017·International Journal of Molecular Sciences·Robert M Hoffman
Jul 14, 2005·Applied Microbiology and Biotechnology·Tomoaki TakakuraAkio Takimoto
Jun 25, 2010·International Journal of Cosmetic Science·N Lourith, M Kanlayavattanakul
Aug 8, 2015·SpringerPlus·Mariusz A Bromke, Holger Hesse
Dec 23, 2015·Biotechnology Research International·Mohsen Helmy SelimAmany Ahmed Hassabo Anise
Sep 25, 2014·BioMed Research International·Bhupender SharmaShamsher S Kanwar
Nov 1, 2016·Applied Biochemistry and Biotechnology·Ashraf S A El-SayedSadik Esener
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Jul 14, 2010·FEMS Microbiology Reviews·Aitor de Las HerasVíctor de Lorenzo
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