Structural analysis of the putative SARS-CoV-2 primase complex.

Journal of Structural Biology
Eva KonkolovaEvzen Boura

Abstract

We report the crystal structure of the SARS-CoV-2 putative primase composed of the nsp7 and nsp8 proteins. We observed a dimer of dimers (2:2 nsp7-nsp8) in the crystallographic asymmetric unit. The structure revealed a fold with a helical core of the heterotetramer formed by both nsp7 and nsp8 that is flanked with two symmetry-related nsp8 β-sheet subdomains. It was also revealed that two hydrophobic interfaces one of approx. 1340 Å2 connects the nsp7 to nsp8 and a second one of approx. 950 Å2 connects the dimers and form the observed heterotetramer. Interestingly, analysis of the surface electrostatic potential revealed a putative RNA binding site that is formed only within the heterotetramer.

Citations

Jul 28, 2020·DNA and Cell Biology·Arghavan AsghariNegin Parsamanesh
Nov 22, 2020·Biomolecular NMR Assignments·Marco TonelliKatherine Henzler-Wildman
Dec 3, 2020·Respiratory Research·Meghana RastogiSunit K Singh
Jan 19, 2021·Journal of Basic Microbiology·Swapnil B KadamVitthal T Barvkar
Dec 1, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiao-Huan LiuTao Wang
Mar 5, 2021·Science Advances·Boris KrichelCharlotte Uetrecht
Mar 18, 2021·Bulletin of the National Research Centre·Chidi Edbert DuruAbayomi Emmanuel Adegboyega
Apr 15, 2021·Expert Review of Molecular Diagnostics·Davide ZellaMassimo Ciccozzi
May 28, 2021·Frontiers in Molecular Biosciences·Nadide AltincekicAndreas Schlundt
May 21, 2021·Journal of Virology·Pavel DostalikEvzen Boura
Jun 15, 2021·Journal of the American Society for Mass Spectrometry·Valentine V CouroublePatrick R Griffin
Jun 16, 2021·Current Opinion in Virology·Robert M Cox, Richard K Plemper
Jul 27, 2021·World Journal of Clinical Cases·Ceylan Polat, Koray Ergunay
Jul 3, 2021·Chemical Society Reviews·Eugene N MuratovAlexander Tropsha
Jul 2, 2021·Biophysical Journal·Mateusz WilamowskiGreg L Hura
Aug 29, 2021·Viruses·Alice-Roza ErueraVernon K Ward
Nov 17, 2021·MBio·Benjamin L SpringsteinAnn Hochschild

❮ Previous
Next ❯

Datasets Mentioned

BETA
AAY32594

Methods Mentioned

BETA
size exclusion chromatography

Software Mentioned

ClustalW
Coot
refine
Phenix
XDS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.