PMID: 3758081Sep 15, 1986Paper

Structural analysis of the sequence coding for an inducible 26-kDa protein in human fibroblasts

European Journal of Biochemistry
G HaegemanW Fiers

Abstract

When human fibroblast cells were stimulated with poly(I) X poly(C) in the presence of cycloheximide for the production of interferon-beta (IFN-beta), a 26-kDa protein could be immunoprecipitated by antiserum raised against partially purified human IFN-beta [Content, J., De Wit, L., Pierard, D., Derynck, R., De Clercq, E. & Fiers, W. (1982) Proc. Natl Acad. Sci. USA 79, 2768-2772]. In our hands this 26-kDa protein showed no antiviral activity. Other investigators have, however, reported the presence in the same conditions of a second type of IFN, a so-called beta 2 species [Weissenbach, J., Chernajovsky, Y., Zeevi, M., Shulman, L., Soreq, H., Nir, U., Wallach, D., Perricaudet, M., Tiollais, P. & Revel, M. (1980) Proc. Natl Acad. Sci. USA 77, 7152-7156] of which the mRNA structure and protein characteristics strongly suggests identity with the 26-kDa product. In this paper we describe the nucleotide sequence of the 26-kDa cDNA and part of the corresponding genomic clone. The cDNA clones were isolated from a library made with mRNA from induced human fibroblasts. As, however, the information thus obtained was still incomplete, genomic clones were isolated from a total human DNA library. In this way, the entire region coding for the...Continue Reading

References

May 4, 1976·Biochemistry·J R LillehaugK Kleppe
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R BreathnachP Chambon
Mar 22, 1985·Science·D J Lipman, W R Pearson
Jan 10, 1986·Nucleic Acids Research·D G GeorgeL T Hunt
Nov 7, 1985·Nature·J M Claverie, I Sauvaget
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N B Raj, P M Pitha
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J WeissenbachM Revel
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H SoreqP B Sehgal
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Jan 11, 1982·Nucleic Acids Research·M I Kanehisa
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J ContentW Fiers
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Jan 22, 1982·Nucleic Acids Research·S M Mount

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Citations

Apr 1, 1991·Journal of Cellular Biochemistry·J KruegerP B Sehgal
Jan 1, 1995·Journal of Clinical Laboratory Analysis·N G PapadopoulosM Papamichail
May 1, 1997·Protein Science : a Publication of the Protein Society·R J SimpsonL D Ward
Jan 1, 1990·International Journal of Cell Cloning·T HiranoT Kishimoto
May 1, 1991·International Journal of Cell Cloning·T Hirano
Jan 1, 1987·Rheumatology International·R Bataille, B Klein
Dec 1, 1989·Cytotechnology·J H Shieh, M A Moore
Feb 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H Baumann
Mar 1, 1996·Immunopharmacology·W Scholz
Mar 1, 1990·Journal of Reproductive Immunology·S SaitoM Ichijo
May 1, 1988·Immunology Today·G G Wong, S C Clark
Dec 1, 1990·Immunology Today·T HiranoT Kishimoto
May 1, 1992·International Journal of Immunopharmacology·P Theisen-PoppR Müller-Peddinghaus
Feb 27, 1995·Molecular and Cellular Endocrinology·H HakovirtaM Parvinen
May 1, 1988·Annales De L'Institut Pasteur. Immunology·J VilcekJ Le
Mar 3, 1998·Critical Reviews in Oncology/hematology·L M TuytE Vellenga
Jun 10, 1998·Cytokine & Growth Factor Reviews·T HiranoM Hibi
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P B SehgalI Tamm
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P DefilippiJ Content
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R D GarmanD H Raulet
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K IkebuchiM Ogawa
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B J RollinsC D Stiles
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M LeJ Vilcek
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T M Rose, A G Bruce
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·K De BosscherG Haegeman
Feb 1, 1988·The Journal of Experimental Medicine·A MuraguchiT Kishimoto
Jun 29, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·T HattoriK Onozaki

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