Structural analysis of UDP-sugar binding to UDP-galactose 4-epimerase from Escherichia coli

Biochemistry
J B ThodenH M Holden

Abstract

UDP-galactose 4-epimerase from Escherichia coli catalyzes the interconversion of UDP-galactose and UDP-glucose through the transient reduction of the tightly bound cofactor NAD+. The enzyme is unique among the NAD+-dependent enzymes in that it promotes stereospecific reduction of the cofactor but nonstereospecific hydride return during normal catalysis. In addition to hydride transfer, the reaction mechanism of epimerase involves two key features: the abstraction of a proton from the 4'-hydroxyl group of glucose or galactose by an active site base and the rotation of a 4-ketopyranose intermediate in the active site pocket. To address the second issue of movement within the active site, the X-ray structures of reduced epimerase complexed with UDP-mannose, UDP-4-deoxy-4-fluoro-alpha-D-galactose, or UDP-4-deoxy-4-fluoro-alpha-D-glucose have been determined and refined to 1.65, 1.8, and 1.65 A resolution, respectively. A comparison of these models to that of the previously determined epimerase/NADH/UDP-glucose abortive complex reveals that the active site accommodates the various sugars by simple rearrangements of water molecules rather than by large changes in side chain conformations. In fact, the polypeptide chains for all of th...Continue Reading

Citations

Sep 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Diro Terefe, Turan Tatlioglu
Aug 1, 2009·Applied Microbiology and Biotechnology·Paul Schweiger, Uwe Deppenmeier
Dec 20, 2012·Applied Microbiology and Biotechnology·Koen BeerensTom Desmet
Oct 14, 2004·Functional & Integrative Genomics·Daniel L E WatersRobert J Henry
Jun 3, 1999·Biochimica Et Biophysica Acta·K FujimotoS Katoh
Jan 24, 2013·Accounts of Chemical Research·Perry A Frey, Adrian D Hegeman
Aug 29, 2006·Chemical Society Reviews·James H Naismith
Jun 13, 2012·Natural Product Reports·Shanteri SinghJon S Thorson
Feb 24, 2001·The Plant Journal : for Cell and Molecular Biology·E T JohnsonG Choi
Sep 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Magnus S AlpheyWilliam N Hunter
Aug 23, 2002·Protein Science : a Publication of the Protein Society·Christopher A BottomsJohn J Tanner
Jan 27, 2009·Applied and Environmental Microbiology·Katarzyna A KazimierczakRustam I Aminov
Jun 12, 2002·Journal of Bacteriology·Christopher K RaymondMaynard V Olson
Oct 4, 2007·PloS One·Gordon V LouieJoseph P Noel
Nov 26, 2008·Chemico-biological Interactions·Rob Meijers, Eila Cedergren-Zeppezauer
Nov 27, 2007·Journal of Molecular Biology·Angelo D FaviaJanet M Thornton
Jun 22, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keita AokiHironori Niki
May 28, 2010·Protein Science : a Publication of the Protein Society·Thomas KowatzJames H Naismith
Aug 12, 2003·Journal of Molecular Biology·Gail J BartlettJanet M Thornton
Jul 15, 2015·Carbohydrate Research·Koen BeerensTom Desmet

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.