Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis

The Journal of Biological Chemistry
Julian P VivianMartin J Scanlon

Abstract

DsbA is an enzyme found in the periplasm of Gram-negative bacteria that catalyzes the formation of disulfide bonds in a diverse array of protein substrates, many of which are involved in bacterial pathogenesis. Although most bacteria possess only a single essential DsbA, Neisseria meningitidis is unusual in that it possesses three DsbAs, although the reason for this additional redundancy is unclear. Two of these N. meningitidis enzymes (NmDsbA1 and NmDsbA2) play an important role in meningococcal attachment to human epithelial cells, whereas NmDsbA3 is considered to have a narrow substrate repertoire. To begin to address the role of DsbAs in the pathogenesis of N. meningitidis, we have determined the structure of NmDsbA3 to 2.3-A resolution. Although the sequence identity between NmDsbA3 and other DsbAs is low, the NmDsbA3 structure adopted a DsbA-like fold. Consistent with this finding, we demonstrated that NmDsbA3 acts as a thiol-disulfide oxidoreductase in vitro and is reoxidized by Escherichia coli DsbB (EcDsbB). However, pronounced differences in the structures between DsbA3 and EcDsbA, which are clustered around the active site of the enzyme, suggested a structural basis for the unusual substrate specificity that is obser...Continue Reading

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J A Peek, R K Taylor
Jan 1, 1986·Methods in Enzymology·R M Macnab
Apr 1, 1981·The Journal of Infectious Diseases·D S Stephens, Z A McGee
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D MissiakasS Raina
Mar 15, 1995·Structure·J L Martin
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F E Dailey, H C Berg
Apr 25, 1997·Journal of Molecular Biology·S H HuJ L Martin
May 30, 1998·The Journal of Biological Chemistry·M BaderJ Bardwell
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Jun 30, 2001·Journal of Biomolecular NMR·J MarleyC Bracken
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·A A Vagin, M N Isupov
Mar 28, 2003·The EMBO Journal·Abhijit A SardesaiCosta Georgopoulos
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Nov 18, 2004·Biochimica Et Biophysica Acta·Hitoshi Nakamoto, James C A Bardwell
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
May 26, 2005·Protein Expression and Purification·F William Studier
Sep 24, 2005·Archives of Microbiology·Alexis DumoulinBrigitte Berger-Bächi
Jul 3, 2007·Lancet·David S StephensPetter Brandtzaeg
Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Annie HinikerJames C A Bardwell
Dec 14, 2007·The Journal of Biological Chemistry·Begoña HerasJennifer L Martin
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Feb 10, 2009·Nature Reviews. Microbiology·Begoña HerasJennifer L Martin
Feb 9, 2010·Antioxidants & Redox Signaling·Goran Malojcić, Rudi Glockshuber
Jan 19, 2011·Antioxidants & Redox Signaling·Stephen R ShouldiceJennifer L Martin
Jul 2, 2009·Journal of Biochemistry·Kenji Inaba
May 7, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·R JarrottB Heras
Mar 7, 2009·Antioxidants & Redox Signaling·Mareike KurzJennifer L Martin
Oct 10, 2009·Journal of Molecular Biology·Julian P VivianMartin J Scanlon
Dec 15, 2017·Antioxidants & Redox Signaling·Makrina TotsikaBegoña Heras
Mar 18, 2010·The Journal of Biological Chemistry·Begoña HerasMark A Schembri

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.